Skip to Content
Merck
All Photos(1)

Documents

5.32280

Sigma-Aldrich

Myeloperoxidase Inhibitor III, HX1

Synonym(s):

Myeloperoxidase Inhibitor III, HX1, MPO Inhibitor III, HX1

Sign Into View Organizational & Contract Pricing


About This Item

Empirical Formula (Hill Notation):
C14H10F6N4O3
CAS Number:
Molecular Weight:
396.24
UNSPSC Code:
12352200

Assay

≥98% (HPLC)

Quality Level

form

powder

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

white

solubility

DMSO: 50 mg/mL

storage temp.

2-8°C

General description

A cell-permeable, trifluoromethyl substituted aromatic hydroxamate compound that acts as a highly potent, selective, mixed type, reversible inhibitor of the chlorination activity of myeloperoxidase (MPO; IC50 = 5 nM). Tightly binds within the active site cavity above the heme and blocks the substrate channel. Displays over 300-fold greater selectivity for MPO when tested against a panel of redox enzymes, including lactoperoxidase (IC50 = 6.3 µM) and thyroid peroxidase (IC50 = 1.59 µM). Blocks the production of hypochlorous acid by human neutrophils (IC50 = 50 nM) and inhibits the formation of NADH bromohydrin (IC50 = 70 nM with 50 µM H2O2 and 10 mM NaBr).
A cell-permeable, trifluoromethyl substituted aromatic hydroxamate compound that acts as a highly potent, selective, mixed type, reversible inhibitor of the chlorination activity of myeloperoxidase (MPO; IC50 = 5 nM). Tightly binds within the active site cavity above the heme and blocks the substrate channel. Displays over 300-fold greater selectivity for MPO when tested against a panel of redox enzymes, including lactoperoxidase (IC50 = 6.3 µM) and thyroid peroxidase (IC50 = 1.59 µM). Blocks the production of hypochlorous acid by human neutrophils (IC50 = 50 nM) and inhibits the formation of NADH bromohydrin (IC50 = 70 nM with 50 µM H2O2 and 10 mM NaBr).

Please note that the molecular weight for this compound is batch-specific due to variable water content.

Biochem/physiol Actions

Cell permeable: yes
Primary Target
MPO

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Forbes, L. V., et al. 2013. J. Bio. Chem.288, 36636.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service