374805

Hemocyanin, Keyhole Limpet, Megathura crenulata

Hemocyanin, Keyhole Limpet, Megathura crenulata, CAS 9013-72-3, is a large, multi-subunit, oxygen-carrying, metalloprotein that is used as a carrier protein in the production of antibodies.

• Synonym(s): Hemocyanin, Keyhole Limpet, Megathura crenulata, KLH
• CAS Number: 9013-72-3
• MDL number: MFCD00131280
• UNSPSC Code: 12352202

Properties

• Quality Level: 200
• Assay: ≥90% (size exclusion chromatography)
• form: lyophilized solid
• does not contain: preservative
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: blue to blue-gray
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Hemocyanin purified from Keyhole Limpet (Mega thura crenulata). Protein:≥60%; copper to protein ratio: ≥1.5 x 10^-3

Protein:≥60%; copper to protein ratio: ≥1.5 x 10^-3

Stable solid containing more than 60% protein. Protein is 90% undissociated hemocyanin.

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from BES buffer, MgSO₄.

Reconstitution

Dissolve at 5-10 mg/ml (protein) in PBS or other physiological buffer by GENTLE addition of the buffer (e.g. slowly added down the side of the tube). DO NOT vortex or agitate vigorously to dissolve product. (KLH is an extremely large protein complex and has a tendency to aggregate and precipitate from solutions.) For further dilutions, use an appropriate buffer and maintain the magnesium concentration at ≥10 mM. Solutions should be stored in the refrigerator (4°C).

Other Notes

Hayashi, K., et al. 1992. Virchows Archiv. B Cell Pathol. 63, 37.
Shuler, K.R., et al. 1992. J. Immunol. Methods 156, 137.
Erlanger, B.F. 1980. Methods Enzymol.70, 85.

Protein concentration: ≥60%

Copper: ≥0.09%

Copper to Protein ratio: ≥1.5 x 10^-3

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3