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1.10338

Millipore

Fractogel® EMD Chelate (M)

purified by immobilized metal affinity chromatography (IMAC)

Synonym(s):

Fractogel® EMD Chelate (M)

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About This Item

UNSPSC Code:
41115711

ligand

iminodiacetic acid

Quality Level

400

description

for metal affinity chromatography (IMAC), suspension in 20% ethanol and 150 mM NaCl (40-90 µm)

sterility

sterile (Caustic Stable)

form

resin

purified by

immobilized metal affinity chromatography (IMAC)

manufacturer/tradename

Fractogel®

parameter

8 bar max. pressure
~240 cm/hr flow rate

matrix active group

methacrylate

wide

40-90 μm

capacity

~60 mg binding capacity( lysozyme/ml of resin)

transition temp

flash point 35 °C (calculated)

application(s)

recombinant protein

separation technique

affinity

storage temp.

2-30°C

General description

Fractogel® EMD Chelate combines our tentacle structure with iminodiacetic acid as the metal chelating ligand

Features and Benefits

Fractogel® EMD Chelate (M) enables users to:

  • Efficiently purify histidine-tagged recombinant proteins
  • Coordinate metal ions to non-bonding electron pairs of the amino acid side chains and separate peptides
  • Ensure timely and successful startup and operation
  • Minimize hydrophobic interactions due to the tentacles technology, resulting in superior selectivity and recovery
  • Handle multiple CIP and packing cycles with the chemically and physically robust bead, leading to a longer resin lifetime

Packaging

  • 1.10338.0100: Fractogel® EMD Chelate (M) Resin 100ml
  • 1.10338.0010: Fractogel® EMD Chelate (M) Resin 10 ml
  • 1.10338.0500: Fractogel® EMD Chelate (M) Resin 500 ml
  • 1.10338.5000: Fractogel® EMD Chelate (M) Resin 5 L

Plastic bottle

Analysis Note

Appearance: Milky tubid suspension, free from impurities (foreigen particles)
Microscopic evaluation: Uniform spherical particles, no agglomerates, no fines
Cerium: ≤ 10 µg/g
Copper-(II)-binding capacity: 60 - 100 µmol/ml
Extractable matter (water): ≤ 0.05 %
Pressure drop(column: ID=1.6 cm, L=10 cm at 5 ml/min): ≤ 1.0 bar
Particle size (d10): 37 - 45 µm
Particle size (d50): 48 - 60 µm
Particle size (d90): 63 - 77 µm
Colony forming units (TAMC + TYMC): ≤ 100 CFU/ml
Endotoxins: ≤ 1.00 EU/ml
Functional test: passes test
Functional test: Separation of chymotrypsinogen A and myoglobin

Legal Information

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

95.0 °F

Flash Point(C)

35 °C

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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