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Millipore

GranuCult® VRBD (Violet Red Bile Dextrose) agar

acc. EP, USP, JP and ISO 21528, For the detection and enumeration of Enterobacteriaceae

Synonym(s):

VRBG Agar. Violet Red Bile Glucose Agar. Crystal-violet neutral-red bile dextrose agar, VRBD-agar (Crystal-violet neutral-red bile dextrose agar) acc. to MOSSEL

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About This Item

UNSPSC Code:
41106213
NACRES:
NA.74

Agency

APHA
EP
ISO 21528
JP
USP

Quality Level

200

sterility

non-sterile

form

granular

manufacturer/tradename

GranuCult® prime

technique(s)

microbiological culture: suitable

pH

7.3 (37 °C, 40 g/L in H2O, after autoclaving)

solubility

39.5 g/L

bulk density

720 kg/m3

application(s)

food and beverages
microbiology
pharmaceutical

storage temp.

15-25°C

suitability

enterobacteriaceae

General description

Violet Red Bile Dextrose Agar is a granulated selective medium recommended for detection and enumeration of Enterobacteriaceae, especially the bile tolerant gram-negative bacteria from non-sterile products and pharmaceutical preparations. The granules ensure rapid and uniform dissolution in water, prevent clumping of the medium, and inhalation of the airborne powder. Pancreatic digest of gelatin and yeast extract provide nitrogenous compounds and other nutrients essential for bacterial metabolism. This media is selective due to the presence of the inhibitors, bile salts, and crystal violet. Crystal violet inhibits gram-positive organisms. The neutral red indicator helps to detect dextrose fermentation. Dextrose fermenting strains produce red colonies with pink-red halos in the presence of neutral red. Sodium chloride maintains the osmotic equilibrium in the medium.

Application

Violet Red Bile Dextrose Agar is recommended for the detection and enumeration of Enterobacteriaceae from pharmaceutical products.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

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Protocols

Revised EN ISO 21528:2017 for Detection and Enumeration of Enterobacteriaceae in the Food Chain

The International Organization for Standardization (ISO) published the revised EN ISO 21528-1 and EN ISO 21528-2 standards in 2017.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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