A reversible inhibitor of trypsin-like proteases and cysteine proteases. Also known to inhibit activation-induced programmed cell death and to restore defective immune response in HIV+ donors. Often used for the inhibition of endoproteinase Lys-C, kallikrein, papain, plasmin, thrombin, cathepsin B, and trypsin. Leupeptin does not inhibit chymotrypsin, elastase, renin, or pepsin. The recommended working range is 10-100 µM for most applications.
Biochem/physiol Actions
Cell permeable: no
Primary Target trypsin- and cysteine-like proteases
Product does not compete with ATP.
Reversible: yes
Warning
Toxicity: Standard Handling (A)
Sequence
Ac-Leu-Leu-arginal, hemisulfate
Reconstitution
Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Aqueous stock solutions are stable for up to 1 week at 4°C or for up to 1 month at -20°C.
Other Notes
Montenez, J.P., et al. 1994. Toxicol. Lett. 73, 201. Sarin, A., et al. 1994. J. Immunol.153, 862.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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