Saltar al contenido
Merck

Inefficient microbial production of refractory dissolved organic matter in the ocean.

Nature communications (2015-06-19)
Helena Osterholz, Jutta Niggemann, Helge-Ansgar Giebel, Meinhard Simon, Thorsten Dittmar
RESUMEN

Dissolved organic matter (DOM) in the oceans constitutes a major carbon pool involved in global biogeochemical cycles. More than 96% of the marine DOM resists microbial degradation for thousands of years. The composition of this refractory DOM (RDOM) exhibits a molecular signature ubiquitously detected in the deep oceans. Surprisingly efficient microbial transformation of labile into stable forms of DOM has been shown previously, implying that microorganisms apparently produce far more RDOM than needed to sustain the global pool. Here we show, by assessing the microbial formation and transformation of DOM in unprecedented molecular detail for 3 years, that most of the microbial DOM is different from RDOM in the ocean. Only <0.4% of the net community production is channelled into a form of DOM that is undistinguishable from oceanic RDOM. Our study provides a molecular background for global models on the production, turnover and accumulation of marine DOM.

MATERIALES
Referencia del producto
Marca
Descripción del producto

Sigma-Aldrich
Ácido clorhídrico solution, 1.0 N, BioReagent, suitable for cell culture
Sigma-Aldrich
Ácido clorhídrico, 36.5-38.0%, BioReagent, for molecular biology
Sigma-Aldrich
L-(−)-Fucosa, ≥98% (GC)
Supelco
Ácido clorhídrico solution, volumetric, 0.1 M HCl (0.1N), endotoxin free
Sigma-Aldrich
D-(+)-Galactosa, ≥99% (HPLC), BioReagent, suitable for cell culture, suitable for insect cell culture
Sigma-Aldrich
Metanol, anhydrous, 99.8%
Sigma-Aldrich
D-(+)-Manosa, powder, BioReagent, suitable for cell culture
Sigma-Aldrich
Hydrogen chloride, ReagentPlus®, ≥99%
Sigma-Aldrich
D-(+)-Manosa, synthetic, ≥99% (GC)
Sigma-Aldrich
Ácido clorhídrico solution, ~6 M in H2O, for amino acid analysis
Sigma-Aldrich
D-(+)-Xylose, ≥99%
Sigma-Aldrich
Cloruro de hidrógeno solution, 3 M in cyclopentyl methyl ether (CPME)
Sigma-Aldrich
D-(+)-Galactosa, ≥99% (HPLC)
Sigma-Aldrich
L-Rhamnose, natural sourced, 99%, FG
Sigma-Aldrich
Ácido clorhídrico solution, 32 wt. % in H2O, FCC
Sigma-Aldrich
Argon, ≥99.998%
Sigma-Aldrich
D-(−)-Arabinose, ≥98% (GC)
Sigma-Aldrich
D-(+)-Manosa, ≥99% (GC), wood
Sigma-Aldrich
D-(+)-Xylose, BioUltra, ≥99.0% (sum of enantiomers, HPLC)
Sigma-Aldrich
D-(+)-Galactosa, ≥98% (HPLC)
Sigma-Aldrich
D-(+)-Xylose, ≥99% (GC)
Sigma-Aldrich
D-(+)-Manosa, BioUltra, ≥99.5% (sum of enantiomers, HPLC)
Sigma-Aldrich
21-Hydroxyprogesterone, ≥97% (HPLC)
Sigma-Aldrich
Octaethylene glycol, ≥95% (oligomer purity)
Sigma-Aldrich
D-(+)-Xylose, ≥99% (GC), BioXtra
Sigma-Aldrich
D-(+)-Galactosa, BioXtra, ≥99% (HPLC)
Sigma-Aldrich
Methanol-12C, 99.95 atom % 12C
Supelco
Methanol solution, contains 0.10 % (v/v) formic acid, UHPLC, suitable for mass spectrometry (MS), ≥99.5%
Sigma-Aldrich
Metanol, HPLC Plus, ≥99.9%, poly-coated bottles
Sigma-Aldrich
D-(+)-Galactosa, meets analytical specification of Ph. Eur., BP