Los valores de pH de todas las disoluciones tampón son dependientes de la temperatura y la concentración. En el caso de los tampones Tris, el pH aumenta alrededor de 0,03 unidades por cada °C de disminución de la temperatura, y disminuye 0,03-0,05 unidades al ser diluido por 10. Para aplicaciones precisas, debe utilizarse un peachímetro cuidadosamente calibrado con un electrodo de vidrio/calomelano combinados.
Legal Information
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
Vetec is a trademark of Merck KGaA, Darmstadt, Germany
Tropical plants have adapted to strong solar ultraviolet (UV) radiation. Here we compare molecular responses of two tropical mangroves Avecennia marina and Rhizophora apiculata to high-dose UV-B. Whole-genome bisulfate sequencing indicates that high UV-B induced comparable hyper- or hypo-methylation in
MicroRNAs (miRNAs) play important roles in plant growth, development, and response to infection by microbes. Double-stranded RNA binding protein 1 (DRB1) facilitates the processing of primary miRNA transcripts into mature miRNAs. Recently, we found that NS3 protein encoded by rice
Here, we describe a method that can be used to evaluate the function of predicted signal peptides. This method utilizes the yeast Saccharomyces cerevisiae YTK12 strain and pSUC2 vector in which the pSUC2 vector with fused predicted signal peptide is
Chao Zhang et al.
Bio-protocol, 8(20), e3060-e3060 (2018-10-20)
Virus inoculation is a basic experimental procedure to evaluate the resistance of a rice variety or a transgenic material upon virus infection. We recently demonstrated that Rice ragged stunt virus (RRSV), an oryzavirus that is transmitted by brown planthopper (BPH)
The content of biogenic compounds and the biological activities of barley (Hordeum vulgare L.)-grain extracts was evaluated. The sufficiently large and heterogeneous set of barley genotypes (100 accessions) enabled the selection of special genotypes interesting for potential industrial, pharmaceutical, and
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
