Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize Ascentis Express column efficiency. The suggested starting point for flow rate for Ascentis Express columns: 1.6 mL/min for 4.6 mm ID; 0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.
53802-U
Ascentis® Express 90 Å C18 (2.7 μm) HPLC Columns
L × I.D. 3 cm × 2.1 mm, HPLC Column
Sinónimos:
Core-shell (SPP) Fused Core C18 HPLC column
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MXP 10,633.00
MXP 10,633.00
Fecha estimada de envío23 de marzo de 2025
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MXP 10,633.00
About This Item
UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
MXP 10,633.00
Fecha estimada de envío23 de marzo de 2025
Productos recomendados
Nombre del producto
Columna para HPLC Ascentis® Express C18, 2,7 μm, 2.7 μm particle size, L × I.D. 3 cm × 2.1 mm
material
stainless steel column
Quality Level
agency
suitable for USP L1
product line
Ascentis®
feature
endcapped
manufacturer/tradename
Ascentis®
packaging
1 ea of
parameter
60 °C temp. range
600 bar max. pressure (9000 psi)
technique(s)
HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
L × I.D.
3 cm × 2.1 mm
surface area
135 m2/g
impurities
<5 ppm metals
matrix
Fused-Core particle platform
superficially porous particle
matrix active group
C18 (octadecyl) phase
particle size
2.7 μm
pore size
90 Å
operating pH
2-9
application(s)
food and beverages
separation technique
reversed phase
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General description
Las columnas de HPLC Ascentis Express, a través del uso de tecnología de partículas Fused-Core®, pueden proporcionarle la elevada velocidad y las elevadas eficiencias de las partículas inferiores a 2 μm a la vez que mantienen menores contrapresiones. La combinación de gran eficiencia y poca contrapresión beneficia a los usuarios de UPLC® (u otro sistema de presión ultraelevada), así como a los usuarios de la HPLC convencional.
Si desea más información sobre esta nueva tecnología de columna, visite la página de inicio Ascentis Express.
Si desea más información sobre esta nueva tecnología de columna, visite la página de inicio Ascentis Express.
Application
Análisis mediante HPLC de las capsaicinas en chile en polvo en Ascentis® Express C18, 2,7 μm
Análisis mediante UHPLC/MS de aceite de maíz en Ascentis® Express C30 en comparación con Ascentis® Express C18
Análisis mediante UHPLC del paracetamol e impurezas en Ascentis® Express C18, 2,7 μm
Análisis mediante HPLC de los parabenos en Ascentis®Express C18, 2,7 μm
Análisis mediante UHPLC/MS de aceite de maíz en Ascentis® Express C30 en comparación con Ascentis® Express C18
Análisis mediante UHPLC del paracetamol e impurezas en Ascentis® Express C18, 2,7 μm
Análisis mediante HPLC de los parabenos en Ascentis®Express C18, 2,7 μm
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
UPLC is a registered trademark of Waters
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Referencia del producto
Descripción
Precios
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Referencia del producto
Descripción
Precios
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Certificados de análisis (COA)
Lot/Batch Number
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Katia Arena et al.
Journal of separation science, 43(9-10), 1781-1789 (2020-04-03)
Olea europaea, meaning "European olive," is a small tree belonging to the family Oleaceae, occurring in the Mediterranean Basin. Olive oil is an essential component of a balanced diet because of its nutritional value. Among micronutrients, phenolic compounds did show important beneficial effects for human health.
Pankaj Partani et al.
Journal of chromatographic science, 54(8), 1385-1396 (2016-05-27)
A liquid chromatography-tandem mass spectrometry method was developed and validated for the simultaneous determination of simvastatin (SV) and simvastatin acid (SVA) in human plasma. To improve assay sensitivity and achieve simultaneous analysis, SVA monitored in (-)ESI (electrospray ionization) mode within
Babu Rao Chandu et al.
SpringerPlus, 2(1), 194-194 (2013-06-07)
A bioequivalence study was proved of generic Febuxostat 80 mg tablets (T) in healthy volunteers.For this purpose, Authors developed a simple, sensitive, selective, rapid, rugged and reproducible liquid chromatography-tandem mass spectrometry method for the quantification of Febuxostat (FB) in human
Petra Šilarová et al.
Food chemistry, 237, 471-480 (2017-08-03)
The degradation of catechins and other phenolics in green tea infusions were monitored using fast HPLC/MS separation. The final separation was performed within 2.5min using Ascentis Express C18 column (50mm×2.1mm i.d.) packed with 2μm porous shell particles. Degradation was studied
Virginia Brighenti et al.
Journal of pharmaceutical and biomedical analysis, 143, 228-236 (2017-06-14)
The present work was aimed at the development and validation of a new, efficient and reliable technique for the analysis of the main non-psychoactive cannabinoids in fibre-type Cannabis sativa L. (hemp) inflorescences belonging to different varieties. This study was designed
Artículos
Derivatization of Aliphatic Amines - Results from the Supelco Ascentis Express column show higher peak resolution, and improved peak shapes than typical chromatograms.
Protocolos
We presents a robust method for analyzing 25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 in serum using a multiplexed LC-MS-MS system.
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What flow rate should I use with Ascentis® Express HPLC Columns?
1 answer-
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Do I need special fittings and tubing to connect Ascentis® Express HPLC Columns?
1 answer-
While operating pressures may not exceed the 400 bar (6,000 psi) capability of your traditional instruments, sustained pressures of about 200 bar (3,000 psi) will exceed the recommended pressure for conventional PEEK tubing and fittings at the column inlet. We recommend changing to stainless steel fittings in all high pressure locations and have designed special High Performance HPLC Fittings/Interconnects that will stay tight at pressures of 1,000 bar (15,000 psi) or greater, even when elevated column temperatures are employed.
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Is there anything special I need to do to my HPLC system to use Ascentis Express?
1 answer-
Nothing special is required to use Ascentis Express HPLC columns. To obtain the full benefits of Ascentis Express, one should minimize dispersion in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level. For more information, request Guidelines for Optimizing Systems for Ascentis Express Columns (T407102).
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Can Ascentis® Express HPLC Columns be used for LC-MS?
1 answer-
Express Fused-Core™ particles were designed with LC-MS in mind. Even extremely short column lengths exhibit sufficient plate counts to show high resolving power. The flat van Deemter plots permit resolution to be maintained at very high flow rates to maximize sample throughput. All Ascentis stationary phases have been evaluated for MS compatibility during their development, and the Express phases are no exception. You can expect extremely low column bleed and background while maintaining longest possible column lifetime. A bonus of Ascentis Express columns for high throughput UHPLC and LC-MS is that they are extremely rugged and highly resistant to plugging, a very common failure mode for competitor columns.
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How can I measure my instrument bandwidth (IBW) and determine what Ascentis® Express HPLC Columns can be used with minimal efficiency loss created by too much internal instrument volume?
1 answer-
The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.
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Can I use Ascentis Express on any type of HPLC system?
1 answer-
Ascentis Express HPLC columns are capable of use on standard HPLC systems as well as UHPLC systems. Columns are packed in high pressure hardware capable of withstanding the pressures used in UHPLC systems.
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What is the carbon load for Ascentis® Express C18 HPLC Columns?
1 answer-
Carbon load which is commonly referred to as %Carbon is misleading and not meaningful for Ascentis® Express. Since Fused-Core™ particle is unique in the sense that it has a solid core and porous shell, the %Carbon number can not be compared to traditional porous particles. The carbon coverage for Ascentis® Express C18 is 3.5 micromoles/meter-squared
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Can I use Ascentis Express on a UHPLC system?
1 answer-
Yes. Ascentis Express columns are packed in a way making them suitable for these ultra high pressure instruments. In fact, Ascentis Express outperforms sub-2 μm micron columns on many applications since Ascentis Express provides the benefits of sub-2 μm particles but at much lower back pressure. These benefits include the capability of providing fast HPLC and higher resolution chromatography. The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell. A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
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