W4769

Monoclonal Anti-WIPI-1 antibody produced in mouse

~1.5 mg/mL, clone WIPI1-4, purified immunoglobulin, buffered aqueous solution

• Sinónimos: Anti-ATG18, Anti-WIPI-1α, Anti-WIPI49
• UNSPSC Code: 12352203

Propiedades

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: WIPI1-4, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~49 kDa
• species reactivity: human, mouse, bovine, rat
• concentration: ~1.5 mg/mL
• technique(s): western blot: 2-4 μg/mL using whole extract of G361 cells
• UniProt accession no.: Q5MNZ9
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... WIPI1(55062) ; mouse ... Wipi1(52639) ; rat ... Wipi1(303630)

Descripción

General description

Monoclonal Anti-WIPI-1 (mouse IgG1 isotype) is derived from the hybridoma WIPI1-4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human WIPI-1, conjugated to KLH. WIPI-1 (WD repeat domain phosphoinositide-interacting protein 1) the mammalian orthologue of Atg18 in S. cerevisiae and A. thaliana, is a member of the WIPI subfamily of WD-repeat (Trp-Asp) proteins. WIPI-1 has a 7-bladed propeller structure and contains a conserved motif for interaction with phospholipids. WIPI-1 is expressed in normal human tissues with high levels in skeletal muscle, heart and testis.

Application

Monoclonal Anti-WIPI-1 antibody has been used in immunoblotting, immunoprecipitation.

Biochem/physiol Actions

WIPI-1 (WD repeat domain phosphoinositide-interacting protein 1) plays an important role in signal transduction, transcription regulation and apoptosis. Endogenous WIPI-1 partially colocalizes with the autophagosomal marker LC3 and induction of autophagy leads to the accumulation of WIPI-1 in large vesicular and cup-shaped structures that are characteristic for autophagy-linked proteins. WIPI-1 is aberrantly expressed in human cancer and is linked to starvation induced autophagy.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.