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T1819

Sigma-Aldrich

Trizma® hydrochloride solution

Trizma® hydrochloride solution
1 of 1 reviewers received a sample product or took part in a promotion

pH 7.0, BioPerformance Certified, 1 M, suitable for cell culture

Sinónimos:

Tris hydrochloride solution

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100 ML
MXP 1,034.00
1 L
MXP 1,898.00

MXP 1,034.00


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100 ML
MXP 1,034.00
1 L
MXP 1,898.00

About This Item

Código UNSPSC:
12161700
NACRES:
NA.25

MXP 1,034.00


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grado

BioPerformance Certified
Molecular Biology
for molecular biology

Nivel de calidad

Formulario

liquid

concentración

1 M

técnicas

cell culture | mammalian: suitable

impurezas

DNase, RNase, Protease, none detected
bioburden, tested
endotoxin, tested
≤5 ppm Heavy metals (as Pb)

pH

7.0

intervalo de pH útil

7.0-9.0

Absorción

≤0.05 at 290 at 40%

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Descripción general

A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.
Tris(hydroxymethyl)aminomethane (Tris) is a standard buffer in biological sciences and has a pH buffering range between 7.0 - 9.0.[1] It is applicable in electrophoretic studies and capillary electrochromatography.[2]

Aplicación

Trizma® hydrochloride solution has been used:
  • as a buffer in reverse transcription and DNase I treatment reactions for RNA isolated from Toxoplasma gondii[3]
  • in the preparation of tris- ethylenediaminetetraacetic acid (EDTA) (TE) buffer for bacterial pellet resuspension for plasmid isolation studies[4]
  • for polylactic acid nanoparticle resuspension, storage, and pH-based stability studies[5]

Información legal

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


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Certificados de análisis (COA)

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A review of acellular immersion tests on bioactive glasses-influence of medium on ion release and apatite formation
Nommeots-Nomm A, et al.
International Journal of Applied Glass Science, 11(3) (11)
Junya Yamagish et al.
Methods in molecular biology (Clifton, N.J.), 1201, 193-205 (2014-11-13)
Parasites change their transcriptional systems in different developmental stages and in response to environmental changes. To investigate the molecular mechanisms that underlie transcriptional regulation, it is essential to identify the exact positions of the transcriptional start sites (TSSs) and characterize
Production and characterisation of biodegradable PLA nanoparticles loaded with thymol to improve its antimicrobial effect
Marcet I, et al.
Journal of Food Engineering, 239 (239)
Kaichun Zhu et al.
Nature protocols, 1(6), 3088-3093 (2007-04-05)
This protocol describes a streamlined method of plasmid DNA extraction by continual thermal lysis, a modification of the basic boiling lysis technique, to simplify the processing of large volumes of Escherichia coli cultures. Fermented bacteria are harvested using a hollow
(Un) suitability of the use of pH buffers in biological, biochemical and environmental studies and their interaction with metal ions-a review
Ferreira CMH, et al.
Royal Society of Chemistry Advances, 5(39) (5)

Questions

  1. What are the storage conditions for this product?

    1 answer
    1. This product is to be stored in room temperature. Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage.

      For more safety and handling information, please view the product Safety Data Sheet:
      https://www.sigmaaldrich.com/sds/sigma/t1819

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Reviews

1 of 1 reviewers received a sample product or took part in a promotion

Active Filters

    • Review 1
    • Votes 0
    4 out of 5 stars.

    NaOCl Sodium Hypochlorite

    All good, however, the delivery time was longer than expected.

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