SRP6451

Fc gamma RIIIB/CD16b human

recombinant, expressed in HEK 293 cells, ≥98% (SDS-PAGE)

• Sinónimos: CD16B, FCG3, FCG3B, FCGR3, FCGR3B, IGFR3
• UNSPSC Code: 51111800
• NACRES: NA.32

Propiedades

• biological source: human
• recombinant: expressed in HEK 293 cells
• tag: 6-His tagged (C-terminus)
• assay: ≥98% (SDS-PAGE)
• form: lyophilized
• mol wt: calculated mol wt 21.7 kDa; observed mol wt 38-50 kDa (DTT-reduced. Protein migrates due to glycosylation. Gly 17 is the predicted N-terminal.)
• packaging: pkg of 10 and 50 μg
• impurities: <1 EU/μg endotoxin (LAL test)
• UniProt accession no.: O75015
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... FCGR3B(2215)

Descripción

General description

Cluster of differentiation 16 (CD16) is a low affinity type 1 transmembrane Fc receptor. It possesses two Ig-like extracellular domains.[1] Cd16 has been identified as two isoforms, Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b). These receptors bind to the Fc portion of IgG antibodies. CD16 encoded by two different highly homologous genes in a cell type-specific manner. CD16 is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16B also known as FCGR3B and FCG3B, is expressed specifically by polymorphonuclear leukocytes (neutrophils) and stimulated eosinophils.[2]

Biochem/physiol Actions

Cluster of differentiation 16B (CD16B) or Fcγ RIIIB (FcγRIIIb) is the low affinity receptor for the Fc region of immunoglobulin γ. It binds complexed or aggregated IgG and also to monomeric IgG. Contrary to CD16A, CD16B is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. CD16B may serve as a trap for immune complexes in the peripheral circulation, which does not activate neutrophils.[2]

Physical form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable