SRP5281

SPHK1, active, GST tagged human

PRECISIO^®, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Sinónimos: SPHK
• UNSPSC Code: 12352202
• NACRES: NA.32

Propiedades

• recombinant: expressed in baculovirus infected Sf9 cells
• product line: PRECISIO^®
• assay: ≥70% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• specific activity: 465-592 nmol/min·mg
• mol wt: ~71 kDa
• NCBI accession no.: BC008040
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... SPHK1(8877)

Descripción

General description

SPHK1 catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (SPP) which is a novel lipid messenger with both intracellular and extracellular functions. Various stimuli increase cellular levels of SPP by activation of SPHK1. Competitive inhibitors of SPHK1 block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors. SPHK1/sphingosine-1-phosphate pathway contributes to colon carcinogenesis. SPHK1 plays a key role in the generation and release of proinflammatory mediators triggered by anaphylatoxins from human macrophages and also is involved in the process of immune cell motility.

Physical form

Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.

Preparation Note

After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable