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SRP5094

Sigma-Aldrich

TSSK1B, active, GST tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Sinónimos:

FKSG81, SPOGA4, STK22D, TSSK1

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.77

recombinant

expressed in baculovirus infected Sf9 cells

product line

PRECISIO® Kinase

assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

51-69 nmol/min·mg

mol wt

~71 kDa

NCBI accession no.

NM_032028

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... TSSK1B(83942)

General description

TSSK1B (testis-specific serine kinase 1B) is a member of a unique subfamily of protein kinases belonging to the AMPK branch on the human kinome tree. TSSK1B is a serine/threonine kinases that is highly expressed in testis and lower express in pancreas, and weak expression in a few other tissues. TSSK members are present in the equatorial segment of human sperm. TSSK1B share 72% amino acid identity and 83% identity in the kinase domain with other family TSSK members and the TSSK1 gene is intronless. In vitro kinase assay shows phosphorylation of TSKS by TSSK1 and TSSK2.

Physical form

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Preparation Note

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Zhonglin Hao et al.
Molecular human reproduction, 10(6), 433-444 (2004-03-27)
Two members of the human testis-specific serine/threonine (Ser/Thr) kinase family, TSSK 1 and TSSK 2, were cloned and sequenced from a human testis adaptor-ligated cDNA library using a PCR strategy. Within the cDNA, open reading frames (ORF) were defined encoding
B Xu et al.
Society of Reproduction and Fertility supplement, 63, 87-101 (2007-06-15)
A family of testis specific serine/threonine kinases, TSSK1-4 and SSTK, in addition to the substrate of TSSK1 & 2, TSKS, have been studied during the past several years in our laboratory. This paper will provide a general background on these

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