SRP4596
IFN-alpha 1 human
recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)
Sinónimos:
IFN-α 1, IFNA1, Interferon-α
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About This Item
Productos recomendados
biological source
human
recombinant
expressed in E. coli
assay
≥95% (HPLC)
≥95% (SDS-PAGE)
form
lyophilized
potency
1x10^8 units/mg
mol wt
~19.3 kDa
packaging
pkg of 100 μg
storage condition
avoid repeated freeze/thaw cycles
impurities
endotoxin, tested
NCBI accession no.
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... IFNA1(3439)
General description
IFN-α1 (interferon α 1) is a type 1 interferon. Human IFN-a1 is a 19.3kDa protein containing 166 amino acid residues. IFN-α1 belongs to the type I interferon (IFN) family and is present in leukocytes. The IFN-α1 gene is mapped to human chromosome 9p21.3.
Application
IFN-alpha 1 human has been used:
- to treat Vero and breast cancer (MDA-MB-231) cells in interferon assay before viral infection
- as a ligand to vaccinia virus B18R protein in analytical size exclusion chromatography
- to treat chikungunya infected human embryonic kidney cell line (HEK293T) to test its effect on Signal transducer and activator of transcription 1 (STAT1) phosphorylation
- as recombinant interferon to treat lung fibroblast cell line (IMR90) to monitor histone chaperone (HIRA) response
Biochem/physiol Actions
IFN-α1 (interferon α 1) is a lymphoid factor that possesses potent anti-viral activity. IFN-α1 interacts with IFNAR1 (interferon α receptor 1) and IFNAR2. The interaction activates JAK1 (janus-activated kinase 1) and tyrosine kinase 2. These events phosphorylate and activate STAT1 and STAT2 (signal transducer and activator of transcription proteins), leading to the upregulation of interferon-stimulated genes some of which encode antiviral proteins. IFN-alpha 1 (IFN-α1) mediates apoptosis in specific tissues. It may be useful in treating laryngeal carcinoma. The interaction of microRNA, miR-181b with IFN-α1, is regarded as a risk factor in systemic lupus erythematosus.
Physical form
Lyophilized without any additives.
Reconstitution
Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.
Reconstitute in water to a concentration of 0.1-1 mg/mL. This solution can then be diluted into other aqueous buffers.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Zoon KC, et al.
The Journal of Biological Chemistry, 267, 15210-15216 (1992)
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Oncotarget, 6(19), 17805-17816 (2015-07-15)
Glioblastomas (GBMs) are deadly tumors of the central nervous system. Most GBM exhibit homozygous deletions of the CDKN2A and CDKN2B tumor suppressors at 9p21.3, although loss of CDKN2A/B alone is insufficient to drive gliomagenesis. MIR31HG, which encodes microRNA-31 (miR-31), is
K Oritani et al.
Cytokine & growth factor reviews, 12(4), 337-348 (2001-09-07)
The type I interferon (IFN) family includes IFN-alpha, IFN-beta, IFN-pi, and IFN-tau. These molecules are clustered according to sequence homologies, use of the same cell surface receptor, and similar functions. IFN-alpha and IFN-beta have a globular structure composed of five
Xiao-Lei Xin et al.
Medical science monitor : international medical journal of experimental and clinical research, 25, 7100-7114 (2019-09-23)
BACKGROUND Interferon alpha (IFNalpha) exerts its anti-proliferative effect on many human cancers. Among the 13 subtypes of human IFNalpha, IFNalpha-1 subtype has 2 variants, named IFNalpha-1a and IFNalpha-1b, that differ from each other in only 1 amino acid, at residue
Yong-Ling He et al.
BioMed research international, 2020, 4757065-4757065 (2020-05-10)
A previous work has discovered that chromosome 1q32 locus linked to the risk of systemic lupus erythematosus (SLE) and miR-181b located on the susceptibility site with downregulation inversely correlating to its target molecular interferon alpha 1 (IFNA1). The purpose of
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