SRP3261

FGF-4 human

Animal-component free, recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)

• Sinónimos: HBGF-4, HST-1, transforming protein KS3
• Código UNSPSC: 12352202
• NACRES: NA.32

Propiedades

• origen biológico: human
• recombinante: expressed in E. coli
• Ensayo: ≥95% (HPLC); ≥95% (SDS-PAGE)
• Formulario: lyophilized
• potencia: ≥0.5 ng/mL
• mol peso: 19.7 kDa
• envase: pkg of 25 μg
• impurezas: <0.1 EU/μg endotoxin, tested
• color: white to off-white
• Nº de acceso UniProt: P08620
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C
• Información sobre el gen: human ... FGF4(2249)

Descripción

Descripción general

The FGF4 (fibroblast growth factor 4) gene is mapped to human chromosome 11q13.3.[1] It is expressed during early embryonic development. In adults, it is strongly expressed in the testis.[2] The encoded protein mainly interacts with FGFR1c (FGF receptor 1c) and FGFR2c. It also binds to FGFR3c and FGFR4, but to a lesser extent.[3]

Aplicación

• FGF-4 human has been used to study the effect of FGF4 on the differentiation of PDMCs (placenta-derived multipotent cells) into hepatocyte-like cells.[4]
• It has been used as a growth factor to study the proliferation and differentiation of human bone marrow mesenchymal stem cells (hMSCs) to chondrocytes.[5]
• It has been used to study hepatic differentiation.[6]

Acciones bioquímicas o fisiológicas

FGF4 (fibroblast growth factor 4) was first identified as an oncogene transforming NIH3T3 (mouse embryo fibroblast cell line) cells. It helps in maintaining the self-renewal capability of normal stem cells (for instance, trophoblastic stem cells). It is also required for cellular differentiation, morphogenesis and proliferation. It controls proliferation and differentiation of normal stem cells, progenitor cells and cancer cells.[2]

Forma física

Lyophilized from 10 mM Sodium Phosphate, pH 7.6 + 100 mM NaCl.

Reconstitución

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable