SRP3196

EGF from mouse

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• Sinónimos: Epidermal Growth Factor, URG, Urogastrone
• Código UNSPSC: 12352202
• NACRES: NA.32

Propiedades

• origen biológico: mouse
• recombinante: expressed in E. coli
• Ensayo: ≥98% (HPLC); ≥98% (SDS-PAGE)
• Formulario: lyophilized
• potencia: 0.1 ng/mL
• mol peso: 6.0 kDa
• envase: pkg of 500 μg
• técnicas: cell culture | mammalian: suitable
• impurezas: <0.1 EU/μg endotoxin, tested
• color: off-white to yellow
• Nº de acceso UniProt: P01132
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C
• Información sobre el gen: mouse ... EGF(13645)

Descripción

Descripción general

EGF (epidermal growth factor) is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells.[1] Recombinant murine EGF is a 6kDa globular protein containing 53 amino acid residues, including 3 intramolecular disulfide-bonds.

Aplicación

EGF from mouse has also been used in the DMEM/F12 media for growing mouse kidney cells.[2]

Acciones bioquímicas o fisiológicas

EGF (epidermal growth factor) has been shown to inhibit gastric secretion, and to be involved in wound healing.[3][4] EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor.[5] This receptor also binds with TGF (transforming growth factor)-α and VGF (vaccinia virus growth factor). EGF plays an important role in embryogenesis and cardiogenesis.[6][7]

Secuencia

NSYPGCPSSY DGYCLNGGVC MHIESLDSYT CNCVIGYSGD RCQTRDLRWW ELR

Forma física

Lyophilized from 10 mM Sodium Phosphate, pH 8.2.

Reconstitución

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.5-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable