SRP3175

TL-1A human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• Sinónimos: TNFSF15, VEGI
• UNSPSC Code: 12352200
• NACRES: NA.32

Propiedades

• biological source: human
• recombinant: expressed in E. coli
• assay: ≥98% (HPLC); ≥98% (SDS-PAGE)
• form: lyophilized
• potency: 10.0-100.0 ng/mL
• mol wt: 22.0 kDa
• packaging: pkg of 20 μg
• technique(s): cell culture | mammalian: suitable
• impurities: <0.1 EU/μg endotoxin, tested
• UniProt accession no.: O95150
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... TNFSF15(9966)

Descripción

General description

TL-1A belongs to the TNF superfamily of ligands. It is expressed predominantly in endothelial cells, and to a lesser extent in placenta, lung, kidney, skeletal muscle, pancreas, small intestine and colon. TL-1A inhibits endothelial cell proliferation and angiogenesis, and has been shown to induce NFkB activation, caspase activity, and apoptosis in responding cell lines. TL-1A interacts with TNFRSF25/DR3 receptor, but can also bind to a decoy receptor TNFRSF21/DR6. Recombinant human TL-1A is a 22.0 kDa polypeptide of 194 amino acid residues.

Biochem/physiol Actions

TL-1A belongs to the TNF superfamily of ligands. Recombinant human TL-1A is a 22.0 kDa polypeptide of 194 amino acid residues.

Sequence

QLRAQGEASV QFQALKGQEF APSHQQVYAP LRADGDKPRA HLTVVRQTPT QHFKNQFPAL HWEHELGLAF TKNRMNYTNK FLLIPESGDY FIYSQVTFRG MTSECSEIRQ AGRPNKPDSI TVVITKVTDS YPEPTQLLMG TKSVCEVGSN WFQPIYLGAM FSLQEGDKLM VNVSDISLVD YTKEDKTFFG AFLL

Physical form

Lyophilized from 10 mM Tris, pH 8.5 + 75 mM Arginine.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable