SRP0143

PRMT4 Active human

recombinant, expressed in baculovirus infected insect cells, ≥65% (SDS-PAGE)

• Sinónimos: Arginine N-methyltransferase 4, CARM1, Coactivator-associated arginine methyltransferase 1, PRMT4
• Código UNSPSC: 12352200
• NACRES: NA.77

Propiedades

• origen biológico: human
• recombinante: expressed in baculovirus infected insect cells
• Ensayo: ≥65% (SDS-PAGE)
• Formulario: aqueous solution
• mol peso: 66 kDa
• envase: pkg of 20 μg
• condiciones de almacenamiento: avoid repeated freeze/thaw cycles
• concentración: >0.02 mg/mL
• Nº de acceso NCBI: NM_199141
• Nº de acceso UniProt: Q86X55
• Condiciones de envío: dry ice
• temp. de almacenamiento: −70°C
• Información sobre el gen: human ... CARM1(10498)

Descripción

Descripción general

Human PRMT4 (CARM1) (GenBank Accession No. NM_199141), amino acids 2-end, with N-terminal His-tag, MW = 66 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Aplicación

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Definición de unidad

One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C.

Forma física

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

Nota de preparación

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.