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Documentos clave

SRE0055

Sigma-Aldrich

Protease Inhibitor Cocktail Powder

Reagents designed and manufactured under cGMP controls suitable for use in an IVD application.

Sinónimos:

Protease Inhibitor Cocktail powder

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About This Item

Código UNSPSC:
12352200
NACRES:
NA.32

origen biológico

bovine lung
yeast

Formulario

lyophilized powder

temp. de almacenamiento

−20°C

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Especificidad

Inhibits serine, cysteine, and metallo-proteases.

Aplicación

Optimized and tested for general use.
Protease inhibitor cocktail powder has been used in immunoblotting and immunoprecipitation.[1]

Acciones bioquímicas o fisiológicas

This mixture contains individual components, including AEBSF at 2 mM, Aprotinin at 0.3 μM, Bestatin at 116 μM, E-64 at 14 μM, Leupeptin at 1 μM and EDTA at 1 mM. Each component has specific inhibitory properties.

AEBSF and Aprotinin act to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin amongst others. Bestatin inhibits aminpeptidases. E-64 acts against cystein proteases. EDTA is an inhibitor of metalloproteases. Leupeptin acts against both serine and cystein proteases.
Protease helps to break down proteins into soluble peptides and amino acids.[2]

Componentes

1 bottle in serum bottle
Components
AEBSF
Aprotinin
Bestatin
E-64
EDTA
Leupeptin

Cantidad

One bottle makes 100 mL of cocktail, which are sufficient to the inhibition of proteases present in approximately 20 gram of cell extract.
Not all extracts contain the same levels of endogenous enzymes, and it may be necessary to adjust the volume of cocktail required.

Nota de preparación

This product is supplied as a lyophilized powder. One bottle prepares 100 mL of a cocktail solution.

Pictogramas

Exclamation mark

Palabra de señalización

Warning

Frases de peligro

Clasificaciones de peligro

Eye Irrit. 2 - Skin Irrit. 2

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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