SBR00049

FITC Labeled D-Alanine

Suitable for bacterial labeling

• Fórmula empírica (notación de Hill): C₂₄H₁₉N₃O₇S
• Peso molecular: 493.49
• Código UNSPSC: 12352209
• NACRES: NA.24

Propiedades

• Nivel de calidad: 200
• Formulario: solid
• temp. de almacenamiento: −20°C

Descripción

Descripción general

Bacterial Peptidoglycan is a polymer consisting of sugars and amino acids, a mesh-like rigid layer that forms the cell wall. D-Amino Acids (D-AAs) are metabolically incorporated onto the bacterial cell wall by D,D-transpeptidase and/or L,D-transpeptidase. It was recently discovered that those transpeptidases catalyze the metabolic incorporation of exogenous D-AAs with almost no restriction of the side-chain identity. [1] Modification of a D-AA with molecular fluorophores provides fluorescent d-amino acids that can efficiently label in situ peptidoglycan in diverse bacterial species. [2]
Fluorescein Labeled D-Alanine is a fluorescent derivative of D-alanine that is obtained by a synthetic conjugation of 3-amino-D-alanine to a fluorophore. Fluorescein Labeled D-Alanine has high biocompatibility and suitability for labeling peptidoglycans in live bacteria

Aplicación

Fluorescent labeled D-AAs can be used for many applications including:

• Bacterial cell wall morphology
• Bacterial cell wall formation or remodeling activity
• Bacterial viability/activity
• Identify bacterial activity on surfaces or in substances

[3] [4]

Nota de análisis

• Fluorescent microscopy application: Fluorescein Labeled D-Alanine has excitation/emission wavelength range at 495 nm/515 nm.
• The recommended working concentration in fluorescent microscopy imaging application is between 250 μM-500 μM in DMSO.
• Aliquots of the DMSO solution can be stored at -20°C, protected from light for at least one month.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable