SAB4200600

Monoclonal Anti-KHSRP antibody produced in mouse

clone KS-8, purified immunoglobulin

• Sinónimos: Anti-6330409F21Rik, Anti-FUBP2, Anti-FBP2, Anti-KH-type splicing regulatory protein, Anti-KHSRP, Anti-KSRP, Anti-MARTA1, Anti-MGC99676
• UNSPSC Code: 12352203
• NACRES: NA.41

Propiedades

• biological source: mouse
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: KS-8, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~82 kDa
• species reactivity: rat, mouse, human, chicken, bovine, canine, hamster
• concentration: ~1.0 mg/mL
• technique(s): flow cytometry: 2-4 μg/test using HEK-293T cells; immunoblotting: 0.25-0.5 μg/mL using HEK-293T total cell extracts; immunocytochemistry: suitable; immunofluorescence: 2.5-5 μg/mL using HEK-293T cells
• isotype: IgG1
• UniProt accession no.: Q92945
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... KHSRP(8570)

Descripción

General description

Monoclonal Anti-KHSRP (mouse IgG1 isotype) is derived from the hybridoma KS-8 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. K homology type-splicing regulatory protein (KHSRP) is an nucleic acid binding protein. It is localized in the cytoplasm and nucleus of the cell. It contains four K homology RNA-binding domains, two N- and C-terminals and protein interaction motifs. KHSRP gene is located on the human chromosome at 19p13.3.

Specificity

Monoclonal Anti- KHSRP recognizes human, cow, chicken, dog, hamster, rat and mouse KHSRP.

Immunogen

synthetic peptide corresponding to an internal sequence of human KHSRP

Application

Monoclonal Anti-KHSRP antibody produced in mouse may be used in:

• immunoblotting
• immunocytochemistry
• flow cytometry
• immunofluorescence

Biochem/physiol Actions

K homology type-splicing regulatory protein (KHSRP) acts as a transcription factor that affects RNA fates at multiple levels including mRNA decay and alternative pre-mRNA splicing. It is involved in rapid decay of AU-rich element (ARE)-containing mRNAs and maturation of microRNAs (miRNAs). KHSRP is significantly modulated in response to breast cancer type 1 (BRCA1) expression, suggesting this factor may contribute to the BRCA1-associated tumor phenotype.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium.

Storage and Stability

For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable