SAB4200247

Anti-GP1BA antibody, Mouse monoclonal

clone PM6/40, purified from hybridoma cell culture

• Sinónimos: MonoclonalAnti-BSS, MonoclonalAnti-CD42B, MonoclonalAnti-CD42balpha, MonoclonalAnti-GP1B, MonoclonalAnti-MGC34595, MonoclonalAnti-glycoprotein Ib (platelet), alpha polypeptide
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• conjugado: unconjugated
• forma del anticuerpo: purified from hybridoma cell culture
• tipo de anticuerpo: primary antibodies
• clon: PM6/40, monoclonal
• Formulario: buffered aqueous solution
• mol peso: antigen ~140 kDa
• reactividad de especies: human
• concentración: ~1.0 mg/mL
• técnicas: flow cytometry: 30-60 μg/mL using human platelet; immunohistochemistry: suitable; western blot: suitable
• isotipo: IgG1
• Nº de acceso UniProt: P07359
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... GP1BA(2811)

Descripción

Descripción general

Monoclonal Anti-GP1BA (mouse IgG1 isotype) is derived from the hybridoma PM6/40 produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. GP1BA, also known as glycoprotein Ib (GP Ib) and CD42b, is a platelet surface membrane glycoprotein. GP Ib is a heterodimer, composed of an α and β chain, that are linked by disulfide bonds. The complete receptor complex includes noncovalent association of the α- and β-subunits with platelet glycoprotein IX and platelet glycoprotein V. The glycoprotein Ib platelet subunit α (GP1BA) gene is mapped to human chromosome 17p13.2.

Especificidad

Monoclonal Anti-GP1BA recognizes human GP1BA/CD42b.

Inmunógeno

human platelet membranes.

Aplicación

Anti-GP1BA antibody, Mouse monoclonal is suitable for:

• immunoblotting
• fluorescence-activated cell sorting (FACS)
• immunohistochemistry

Acciones bioquímicas o fisiológicas

Glycoprotein Ib (GP Ib) functions as a receptor for von Willebrand factor (VWF). It regulates the adhesion of blood platelets to damaged blood vessel walls and the subsequent platelet aggregation. It also transmits signals leading to platelet activation, aggregation and secretion. The binding of the GP Ib-IX-V complex to VWF, facilitates initial platelet adhesion to vascular subendothelium after vascular injury, and also initiates signaling events within the platelet that lead to enhanced platelet activation, thrombosis, and hemostasis. The interaction between Gp Ib and VWF was found to induce apoptotic events in platelets, suggesting a mechanism for platelet clearance or some thrombocytopenic disease. Furthermore, several polymorphisms and mutations have been described in this gene, some of which are the cause of Bernard Soulier syndromes and platelet-type von Willebrand disease.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Almacenamiento y estabilidad

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20oC in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable