S4920
SITE Liquid Media Supplement (100×)
liquid, sterile-filtered, BioReagent, suitable for cell culture
Sinónimos:
Media supplement
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5 ML
MXP 1,079.00
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5 ML
MXP 1,079.00
About This Item
Código UNSPSC:
12352205
NACRES:
NA.75
Productos recomendados
esterilidad
sterile-filtered
Línea del producto
BioReagent
Formulario
liquid
técnicas
cell culture | mammalian: suitable
impurezas
endotoxin, tested
Condiciones de envío
ambient
temp. de almacenamiento
2-8°C
Categorías relacionadas
Descripción general
SITE Liquid Media Supplement (100×) serves as a substitute for serum-free formulations. It contains purified factors required for in vitro growth and differentiation studies. The addition of supplements to media will vary, depending on the cell type being studied and the basal medium employed. It is a general cell supplement designed for use in non-complex media (e.g., minimum essential medium (MEM), Roswell Park Memorial Institute Medium (RPMI-1640)) and complex media (e.g., Ham′s F-12, Dulbecco′s Modified Eagle Medium (DME) /F-12, MEM) with sodium pyruvate.
Aplicación
Acciones bioquímicas o fisiológicas
- Insulin: a polypeptide hormone that promotes the uptake of glucose and amino acids and may owe an observed mitogenic effect to this property.[4]
- Transferrin: an iron-transport protein. Iron is an essential trace element but can be toxic in its free form. To nourish cells in culture, iron is supplied bound to transferrin in serum.[5]
- Selenium: an essential trace element normally provided by serum.[6]
- Ethanolamine: plays a significant role in the proliferation of hybridoma cells and frequently is added to supplements used for culturing these cells.[7]
Componentes
Contains 1.0mg/ml recombinant human insulin, 0.55mg/ml human transferrin (substantially iron-free), 0.5μg/ml sodium selenite and 0.2mg/ml ethanolamine at the 100x concentration.
Nota de preparación
Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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