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S1452

Sigma-Aldrich

MISSION® siRNA Transfection Reagent

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MISSION® siRNA Transfection Reagent

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About This Item

UNSPSC Code:
41106502
NACRES:
NA.51

product line

MISSION®

form

aqueous solution

shipped in

wet ice

storage temp.

2-8°C

Categorías relacionadas

General description

The MISSION® siRNA Transfection Reagent is for the transfection of siRNA to achieve ≥90% silencing efficiency for transient knockdown of eukaryotic gene expression.
Effective gene silencing and reduced off target effects are achieved using a low siRNA concentration (1nM). The siRNA Transfection Reagent is provided as a sterile solution, and is compatible with serum and antibiotics.

Other Notes

Gene silencing assay: An A549 cell line stably expressing firefly luciferase was transfected in 24-well plates with 8.4 ng of 21-mer Luciferase siRNA using 2 μL of the MISSION® siRNA Transfection Reagent. Experiments were performed in triplicate in presence of serum. Cell lysates were assessed for luciferase expression 48 hours post-transfection.

Legal Information

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

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Referencia del producto
Descripción
Precios

08168

Timestrip Plus™ 8 °C

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk_germany

WGK 3

flash_point_f

120.2 °F

flash_point_c

49 °C

Certificados de análisis (COA)

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Tuyen T Dang et al.
Cancers, 12(7) (2020-07-11)
It was recently shown that the 5' to 3' exoribonuclease XRN2 is involved in the DNA damage response. Importantly, loss of XRN2 abrogates DNA double stranded break repair via the non-homologous end-joining pathway. However, the mechanistic details of how XRN2
Ilaria Guerriero et al.
Oncotarget, 8(12), 19172-19191 (2016-11-24)
Hyperactivation of the PI3K/AKT pathway is observed in most human cancer including lung carcinomas. Here we have investigated the role of miRNAs as downstream targets of activated PI3K/AKT signaling in Non Small Cell Lung Cancer (NSCLC). To this aim, miRNA
A E Witt et al.
Oncogene, 36(12), 1707-1720 (2016-10-04)
Tumours are comprised of a highly heterogeneous population of cells, of which only a small subset of stem-like cells possess the ability to regenerate tumours in vivo. These cancer stem cells (CSCs) represent a significant clinical challenge as they are
Ponnuraj Nagendraprabhu et al.
PLoS pathogens, 13(12), e1006779-e1006779 (2017-12-16)
Poxviruses have evolved multiple strategies to subvert signaling by Nuclear Factor κB (NF-κB), a crucial regulator of host innate immune responses. Here, we describe an orf virus (ORFV) virion-associated protein, ORFV119, which inhibits NF-κB signaling very early in infection (≤
Tuyen T Dang et al.
International journal of molecular sciences, 21(12) (2020-06-19)
Cellular survival is dependent on the efficient replication and transmission of genomic information. DNA damage can be introduced into the genome by several different methods, one being the act of DNA replication. Replication is a potent source of DNA damage
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Artículos

How Transfection Works

This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.

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Transfection Reagent Selection Guide

Browse our convenient transfection reagent selection guide to match the best reagent for your specific cell line and application needs.

Expresión y silenciamiento génicos

Sigma-Aldrich® Advanced Genomics es el principal proveedor de tecnologías de edición y silenciamiento de genes, entre ellas, CRISPR, Cas9, ARN guía sintético (sgRNA) y nucleasa con dedos de zinc (ZFN).

Gene Expression & Silencing

Sigma-Aldrich® Advanced Genomics is the leading provider of gene editing and silencing technologies including CRISPR, Cas9, synthetic guide RNA (sgRNA), and Zinc Finger Nuclease (ZFN).

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico