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Merck

RES3098T-B7

SAFC

Clorhidrato de Trizma®

Sinónimos:

Clorhidrato de trometano, TRIS / HCI, TRIS hydrochloride, Tris(hidroximetil)-aminometano hydrochloride

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About This Item

Fórmula lineal:
NH2C(CH2OH)3 · HCl
Número de CAS:
Peso molecular:
157.60
Beilstein/REAXYS Number:
3675235
EC Number:
MDL number:
UNSPSC Code:
12352200
NACRES:
NA.21

biological source

synthetic

form

powder

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, microbial, and trace metals, tested

useful pH range

7.0-9.0

pKa (25 °C)

8.1

suitability

suitable for manufacturing use

foreign activity

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

SMILES string

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

InChI key

QKNYBSVHEMOAJP-UHFFFAOYSA-N

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Application

Tris Hydrochloride is a commonly used biological buffer which is also referred to as Trizma HCl. Tris as a buffer has very broad usage in biological systems such as diagnostics, downstream purification and cell culture. The pKa of Tris is 7.77 which corresponds closely to a physiological pH of 7.36.

Tris HCl s used in biopharmaceutical buffer formulations (both upstream and downstream), electrophoresis separations and diagnostic reagent formulations. Tris HCl is especially useful in downstream purification processes.

Packaging

Product is available in the following package sizes:
RES3098T-B701X: 100g container
RES3098T-B703X: 5kg container
RES3098T-B705X: 25kg container
RES3098T-B706X: 50kg container

Other Notes

Los valores de pH de todas las disoluciones tampón son dependientes de la temperatura y la concentración. En el caso de los tampones Tris, el pH aumenta alrededor de 0,03 unidades por cada °C de disminución de la temperatura, y disminuye 0,03-0,05 unidades al ser diluido por 10. Para aplicaciones precisas, debe utilizarse un peachímetro cuidadosamente calibrado con un electrodo de vidrio/calomelano combinados.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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