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Merck

RAB0480

Sigma-Aldrich

Rat Tumor Necrosis Factor α ELISA Kit

for cell and tissue lysates

Sinónimos:

Cachectin, TNF-alpha, Tumor necrosis factor alpha

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1 KIT
MXP 12,604.00

MXP 12,604.00


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1 KIT
MXP 12,604.00

About This Item

Código UNSPSC:
41116158
NACRES:
NA.32

MXP 12,604.00


Check Cart for Availability

reactividad de especies

rat

envase

kit of 96 wells (12 strips x 8 wells)

técnicas

ELISA: suitable
capture ELISA: suitable

entrada

sample type cell lysate
sample type tissue lysate

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 25 pg/mL
standard curve range: 82.3-20000 pg/mL

método de detección

colorimetric

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

Información sobre el gen

rat ... Tnf(24835)

Descripción general

The Rat TNF-α ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat TNF-α cell lysate and tissue lysate.

Inmunógeno

Recombinant Rat TNF-α

Aplicación

For research use only. Not for use in diagnostic procedures.
Rat Tumor Necrosis Factor α ELISA Kit has been used to measure rat tumor necrosis-α (TNF-α) in cell lysate and tissue lysate.[1][2]

Acciones bioquímicas o fisiológicas

Tumor necrosis factor-α (TNF-α) affects various cellular processes such as blood-brain barrier, inflammatory, thrombogenic, and vascular changes following brain damage. TNF-α expression is upregulated in various central nervous system disorders, including Alzheimer′s disease, multiple sclerosis, Parkinson′s disease, meningococcal meningitis and HIV infection. Elevated expression of TNF-α in brain and blood in response to lipopolysaccharide might increase brain stem thrombosis and hemorrhage and stroke sensitivity/risk in hypertensive rats.[3] Tumor necrosis factor-α activates polymorphonuclear leukocyte NADPH oxidase, thereby facilitates development of systemic oxidative stres (OS), inflammation and hypertension in rats.[4]

Otras notas

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS

  • RABDIL6ELISA 5X Sample Diluent Buffer (Item D2)SDS

  • RABELADEELISA 5X Assay/Sample Diluent Buffer E (Item E2)SDS

  • RABLYSIS12X Cell Lysis Buffer (Item J)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

Pictogramas

Corrosion

Palabra de señalización

Warning

Frases de peligro

Consejos de prudencia

Clasificaciones de peligro

Met. Corr. 1

Código de clase de almacenamiento

8A - Combustible corrosive hazardous materials


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Visite la Librería de documentos

Vu Thi Thu et al.
The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology, 20(3), 305-314 (2016-05-11)
Inflammatory and fibrotic responses are accelerated during the reperfusion period, and excessive fibrosis and inflammation contribute to cardiac malfunction. NecroX compounds have been shown to protect the liver and heart from ischemia-reperfusion injury. The aim of this study was to
Nanik Sundari et al.
Journal of toxicology, 2018, 2642714-2642714 (2019-01-12)
Phaleria macrocarpa is one of the Indonesian herbal plants which has been shown to have a hepatoprotective effect. This study was conducted to evaluate the protective effect of water extract of mahkota dewa (Phaleria macrocarpa) in liver fibrosis and to
Tumor necrosis factor-alpha: a possible priming agent for the polymorphonuclear leukocyte-reduced nicotinamide-adenine dinucleotide phosphate oxidase in hypertension.
Mazor R
Hypertension, 55(2), 353-362 (2010)
R A Ajala-Lawal et al.
Archives of physiology and biochemistry, 126(2), 107-115 (2018-10-06)
This study investigated the influence of betulinic acid on high-fructose diet-induced metabolic syndrome in rats. Oral administration of betulinic acid significantly reversed high-fructose diet-mediated increase in body mass index and blood glucose. Furthermore, betulinic acid restored high-fructose diet-mediated alterations in
Meijuan Chen et al.
Gastroenterology research and practice, 2019, 8723460-8723460 (2019-10-01)
Phosphatidylcholine (PC) is the major surface-active phospholipid and creates a hydrophobic nature to the surface. It has been reported to reverse the progression of liver fibrosis and to improve liver function. The aim of the present study was to evaluate

Questions

  1. how many tests can be done by a kit

    1 answer
    1. The Rat TNF-alpha ELISA kit provides one 96-well plate. A set of 7 standards is loaded in duplicates to generate a standard curve, leaving 82 wells for testing 41 samples in duplicates.

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