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RAB0037

Human Procalcitonin ELISA Kit

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1 kit
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MXP 12,305.00

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NACRES:
NA.32
UNSPSC Code:
41116158
eCl@ss:
32160701
Gene information:
human ... CALCA(796)
Input:
sample type cell culture supernatant(s)
sample type urine
Species reactivity:
human
Storage temp.:
−20°C
Shipped in:
wet ice

MXP 12,305.00


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description

for cell culture supernatant and urine. Note :The detection range of this kit is 30 pg/ml - 20000 pg/ml.  Procalcitonin is low in normal serum and may be undetectable with this assay.

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, capture ELISA: suitable

input

sample type cell culture supernatant(s)
sample type urine

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 30 pg/mL
standard curve range: 27.43-20000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... CALCA(796)

General description

The Human Procalcitonin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Procalcitonin in cell culture supernatants and urine. Note: The detection range of this kit is 30 pg/ml - 20000 pg/ml.  Procalcitonin is low in normal serum and may be undetectable with this assay.

Immunogen

Recombinant Human Procalcitonin

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Procalcitonin or calcitonin related polypeptide a is a 116-amino acid precursor of calcitonin. It is secreted by monocytes activated by endotoxins or pathogens. The protein is a biomarker for sepsis and bacterial infections. Procalcitonin has been associated with hepatocyte dysfunction.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

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RAB0407RAB0416RAB1075
description

-

description

serum, plasma, cell culture supernatant and urine

description

for serum, plasma and cell culture supernatant

description

for cell culture supernatants, plasma, and serum samples

species reactivity

human

species reactivity

human

species reactivity

mouse

species reactivity

human

Gene Information

human ... CALCA(796)

Gene Information

human ... PLG(5340)

Gene Information

mouse ... Retn(57264)

Gene Information

human ... POSTN(10631)

input

sample type cell culture supernatant(s)
sample type urine

input

sample type cell culture supernatant(s)
sample type plasma
sample type serum
sample type urine

input

sample type serum
sample type plasma
sample type cell culture supernatant(s)

input

sample type serum sample(s)
sample type cell culture supernatant(s)
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 30 pg/mL
standard curve range: 27.43-20000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 20000 pg/mL
standard curve range: 31.25-2000 ng/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 5 pg/mL
standard curve range: 4.096-1000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 80 pg/ml

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

capture ELISA: suitable

technique(s)

capture ELISA: suitable

technique(s)

ELISA: suitable

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric


Pictogramas

Health hazard

Palabra de advertencia

Danger

Códigos de peligro

Indicaciones de precaución

Hazard Classifications

Resp. Sens. 1

Clase de almacenamiento

10 - Combustible liquids



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Certificados de análisis (COA)

Lot/Batch Number

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Questions

1–2 of 2 Questions  
  1. Can this kit be used to assess procalcitonin levels in human vitreous fluid?

    1 answer
    1. RAB0037 has not been validated for human vistreous fluid. Before using it for vitreous fluid, customer needs to perform validation such as accuracy, precision, dilution linearity; sample dilution and detectability also need to be determined.

      Helpful?

  2. Can the procalcitonin kit used for Human Sputum

    1 answer
    1. This product has not been validated for sputum. For sputum samples, it is recommended to add a protease inhibitor cocktail at a concentration of 1:500. Centrifugation should be performed at 10,000 rpm for 10 minutes, and the supernatant should be diluted 1:2 with sample diluent buffer D prior to assay setup. If necessary, samples can be ultra-centrifuged for 4 hours at 38,000 rpm.

      Helpful?

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