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R7757
Red Blood Cell Lysing Buffer Hybri-Max™
Liquid, sterile-filtered, suitable for hybridoma
Sinónimos:
RBC Lysing Buffer
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100 ML
MXP 697.00
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100 ML
MXP 697.00
About This Item
UNSPSC Code:
12352207
NACRES:
NA.75
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Nombre del producto
Red Blood Cell Lysing Buffer Hybri-Max™, liquid, sterile-filtered, suitable for hybridoma
sterility
sterile-filtered
Quality Level
form
liquid
technique(s)
cell culture | hybridoma: suitable
impurities
endotoxin, tested
Application
Recommended for use by adding 1 mL of buffer to a cell pellet (cell pellet = 1 spleen or 100-200 million cells). Gently mix for 1 minute. Dilute the buffer with 15-20 mL of medium or salt solution. Centrifuge at 250-500 × g for 7 minutes and decant the supernatant. Cells may be diluted and prepared for counting or fusion. If lysis is incomplete, steps 1-4 may be repeated.
Biochem/physiol Actions
Red Blood Cell Lysing Buffer has been developed for use in hybridoma protocols to remove red blood cells from mouse splenocyte suspensions before fusion. It is also useful in systems where it may be desirable to remove red blood cells from cell suspensions, such as whole blood.
Components
Contains 8.3 g/L ammonium chloride in 0.01 M Tris-HCl buffer.
Other Notes
Note: This product is intended for the removal of red blood cells from mice. This product may not be appropriate for the lysis of red blood cells of other animals. The suitability of the product in any application other than mouse splenocytes must be determined by the researcher.
Legal Information
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
Storage Class
12 - Non Combustible Liquids
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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What is the Department of Transportation shipping information for this product?
1 answer-
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Is the BSA standard supplied with Product B6916, Bradford Reagent?
1 answer-
No, we do not supply the BSA standard with this reagent. We recommend using one of the following products: Protein Standard (BSA) Solution, (2 mg/mL), Product No. P0834 or Protein Standard (BSA) Solution, (1 mg/mL), Product No. P0914, if lower concentrations of protein are to be measured.
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Can Product R7757, Red Blood Cell Lysing Buffer Hybri-Max™, be used to lyse cells that are trapped in whole tissue preparations?
1 answer-
Unfortunately, we have not verified the suitability of this lysis buffer for using with tissue samples and do not have a protocol.
Helpful?
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Since you do not recommend Product R7757, Red Blood Cell Lysing Buffer Hybri-Max™, for treating whole blood, what protocol and products do you recommend for red cell lysis in a sample of whole blood?
1 answer-
The following is a suggested protocol:1. Centrifuge to remove plasma. A volume of 0.2 mL of whole blood will yield approximately 0.1 mL of packed cells. Add 0.1ml of 1X buffer such as HBSS (Hanks' balanced salt solution, Product No. H1641) or DPBS (Dulbecco's phosphate buffered saline solution, Product No. D1283), to 0.1 mL of the pelleted cells and resuspend the pellet.2. Add 1-2 mls of 0.1X diluent (or water) and quickly mix cells using the same pipette.3. After 15 seconds, quickly add a volume of 2X diluent in the amount used in step 2 and mix thoroughly. Dilute further with 1X diluent. Wash cells once with buffer.
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In step 3 of your usage sheet for Product R7757, Red Blood Cell Lysing Buffer Hybri-Max™, you say to dilute this buffer with medium or "salt solution". What salt solution is appropriate?
1 answer-
Any balanced salt solution such as DPBS (Dulbecco's phosphate buffered saline) or HBSS (Hank's Balanced Salt Solution) may be used to dilute the buffer when following this procedure.
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