This product is considered salt free. Following purification by Ion exchange chromatography, the material is precipitated in ethanol, washed, and finally dialyzed against water prior to lyophilization.
R1753
Ribonucleic acid, transfer from Escherichia coli
Type XX, Strain W, lyophilized powder
Sinónimos:
Transfer RNA, tRNA
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Application
Ribonucleic acid, transfer from Escherichia coli, Type XX, Strain W may be used as a starting material for the purification of specific amino acyl-tRNA species by methods such as liquid column chromatography or high-performance liquid chromatography (HPLC).
Unit Definition
One unit will yield an A260 of 1.0 in 1.0 mL of water (1 cm light path).
Analysis Note
Each lot is evaluated for its amino acid acceptor activity using aminoacyl-tRNA synthetase and Arg.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Certificados de análisis (COA)
Lot/Batch Number
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Roya Pedram Fatemi et al.
Journal of biomolecular screening, 20(9), 1132-1141 (2015-07-16)
Long non-protein coding RNAs (lncRNAs) are an important class of molecules that help orchestrate key cellular events. Although their functional roles in cells are not well understood, thousands of lncRNAs and a number of possible mechanisms by which they act
Miguel López-Estepa et al.
PloS one, 10(4), e0118606-e0118606 (2015-04-22)
Cyclic N6-threonylcarbamoyladenosine ('cyclic t6A', ct(6)A) is a non-thiolated hypermodification found in transfer RNAs (tRNAs) in bacteria, protists, fungi and plants. In bacteria and yeast cells ct(6)A has been shown to enhance translation fidelity and efficiency of ANN codons by improving
Kirill Kondratov et al.
Journal of extracellular vesicles, 9(1), 1743139-1743139 (2020-04-29)
The aim of this study was to investigate cell source-dependent nucleic acids repertoire of diverse subpopulations of plasma extracellular vesicles (EVs). Blood plasma from nine healthy volunteers was used for the analysis. Samples of EVs were obtained by differential centrifugation
Thomas E Sladewski et al.
eLife, 7 (2018-06-27)
We investigated the role of full-length Drosophila Bicaudal D (BicD) binding partners in dynein-dynactin activation for mRNA transport on microtubules. Full-length BicD robustly activated dynein-dynactin motility only when both the mRNA binding protein Egalitarian (Egl) and K10 mRNA cargo were
Adi Egozi et al.
Cell reports, 32(7), 108043-108043 (2020-08-20)
The islets of Langerhans are dynamic structures that can change in size, number of cells, and molecular function in response to physiological and pathological stress. Molecular cues originating from the surrounding "peri-islet" acinar cells that could facilitate this plasticity have
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R1753 Ribonucleic acid,CAS Number 9014-25-9, transfer from Escherichia coli. Is it desalted?
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R1753 Ribonucleic acid,CAS Number 9014-25-9, transfer from Escherichia coli. Is it purified E. coli tRNA?
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The product is isolated from Escherichia coli. It is soluble in water at 2 mg/ml, producing a clear, colorless solution.
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What is the meaning of "type XX" in R1753?
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There are three classes of RNA: Messenger, Ribosomal, and Transfer. The type designations on products offered by Sigma are in-house designations and do not represent any specific biochemical significance. These designations can refer to the order of addition of the product to the catalog, source of material, method of preparation, differences in specifications, and so on.
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I am confused about this reagent based on the application vs. analysis note. Does this product contain deacylated tRNA or is it a mixture of various aminoacylated tRNAs?
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This product is just deacylated tRNA. It is tested for tRNA for multiple amino acids.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can Product R1753, tRNA, be used in molecular biology applications?
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This product has not been tested for Dnase or RNase activity. Product Nos. R5636 and R8508 are from baker's yeast; they have been tested for DNase and Nickase.
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How many mgs of tRNA, Product R1753, come in the 100UN, 500UN, 2KU and 10KU package sizes?
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The amount of powder will vary between lots depending on the activity. For example, a Certificate of Analysis of a lot may say 18 A260 units/mg solid. This means that the 100UN package will contain approxomately 5.56 mg of solid.
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