The components of this kit are sufficient for 36 assays and include 2-Aminobenzamide, DMSO, Glacial Acetic Acid, and Sodium Cyanoborohydride as a reductant. This kit does not include the clean-up cartridges, product G4548, which are required for use. These are preset quantities. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/610/790/pp0520bul.pdf
PP0520
GlycoProfile™ 2-AB Labeling Kit
Sinónimos:
2-AB Labeling Kit
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Seleccione un Tamaño
1 KIT
MXP 12,293.00
MXP 12,293.00
Fecha estimada de envío27 de marzo de 2025
Seleccione un Tamaño
Cambiar Vistas
1 KIT
MXP 12,293.00
About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
MXP 12,293.00
Fecha estimada de envío27 de marzo de 2025
Productos recomendados
Quality Level
General description
The GlycoProfile™ labeling kits are designed for efficient labeling of N-linked, O-linked, and glycosylphosphatidylinositol (GPI) anchored glycans with either 2-aminobenzamide (2-AB) or 2-aminobenzoic acid (anthranilic acid; 2-AA). These small fluorophores increase the spectral absorption of glycans, improving the detection of labeled glycans by HPAE (high-performance anion exchange chromatography), HPLC, and ESI-MS (electrospray mass spectroscopy) methods. Binding is robust and the resulting dye-glycan conjugates are stable, with no degradation during post-labeling analysis.
Application
GlycoProfile™ 2-AB Labeling Kit has been used for the labelling of N-linked glycans from human IgG, Bovine fetuin, and[1] human epididymis protein 4.[2] It has also been used for labelling free oligosaccharides from mouse spleen.[3]
While 2-AB is slightly less sensitive than 2-AA, it is suitable for downstream glycan analysis by HPAE, HPLC and ESI-MS. Although suitable for the same applications, 2-AA is better suited for SDS-PAGE.
Legal Information
Labeling of glycans with 2-AB is covered under US Patent No. 5,747,347 and its foreign equivalents.
GlycoProfile is a trademark of Sigma-Aldrich Co. LLC
Solo componentes del kit
Referencia del producto
Descripción
- 2-AB Labeling Reagent 2 x 5
- Dimethyl sulfoxide (DMSO) 2 x 350
- Acetic acid, glacial 2 x 200
- Reductant (sodium cyanoborohydride) 2 x 6
signalword
Danger
Hazard Classifications
Acute Tox. 2 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 2 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Sol. 1 - Skin Corr. 1A - Water-react 1
supp_hazards
Storage Class
4.3 - Hazardous materials which set free flammable gases upon contact with water
Elija entre una de las versiones más recientes:
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Los clientes también vieron
Florian Cambay et al.
Current research in immunology, 1, 23-37 (2020-06-27)
The effector functions of the IgGs are modulated by the N-glycosylation of their Fc region. Particularly, the absence of core fucosylation is known to increase the affinity of IgG1s for the Fcγ receptor IIIa expressed by immune cells, in turn
Separation of 2-aminobenzamide labeled glycans using hydrophilic interaction chromatography columns packed with 1.7 mum sorbent
Ahn J, et al.
Journal of Chromatography. B, Biomedical Sciences and Applications, 878(3-4), 403-408 (2010)
Enhancing the antiviral potency of ER alpha-glucosidase inhibitor IHVR-19029 against hemorrhagic fever viruses in vitro and in vivo
Ma J, et al.
Antiviral research, 150, 112-122 (2018)
Expression and biochemical characterization of recombinant human epididymis protein 4
Hua L, et al.
Protein Expression and Purification, 102, 52-62 (2014)
Céline Raymond et al.
mAbs, 7(3), 571-583 (2015-04-16)
The presence of α2,6-sialic acids on the Fc N-glycan provides anti-inflammatory properties to the IgGs through a mechanism that remains unclear. Fc-sialylated IgGs are rare in humans as well as in industrial host cell lines such as Chinese hamster ovary
Artículos
Glycoprofile Labeling Kits efficiently label glycans for glycan analysis, offering flexibility in choice of labeling.
Fast assessment of antibody-dependent cell-mediated cytotoxicity (ADCC) and glycoform pattern of a therapeutic antibody rituximab by FcγRIIIa affinity chromatography.
Glycans play a key role in protein structure and disease; representation on cell surfaces is the glycome.
Protocolos
Uncover how HPLC analysis of glycans helps researchers analyze glycan structures and glycoforms & find products to assist in your glycan analysis methods.
Explore mass spectrometry analysis of glycans for glycomic & glycoproteomic neutral & acidic glycan analysis. See a general mass spec glycan analysis procedure.
-
What is available in the 2-AB labeling kit? Are the weights preset or customer set?
1 answer-
Helpful?
-
Active Filters
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico
