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PNS1010

Sigma-Aldrich

Stabilyser Reagent

for stabilization of DNA, RNA, and protein in tissue extracts

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About This Item

UNSPSC Code:
41116012
NACRES:
NA.77

form

liquid

Quality Level

200

feature

for stabilization of DNA, RNA, and protein in tissue extracts

shipped in

wet ice

storage temp.

2-8°C

General description

The stability of protein and antibody structure and function is dependent on a variety of factors such as temperature, freeze/thaw cycles, proteases and pH. For some proteins and antibodies, function can be lost completely with a single freeze/thaw cycle and proteases can significantly degrade proteins and antibodies within hours even when kept at 4 °C. Stabilyser Reagent minimizes the risk to improve protein assay quality and accuracy.

Stabilyser Reagent is a proprietary formulation of detergents and solutes optimized for efficient extraction, stabilization and storage of protein, DNA, and RNA from tissue samples. The unique composition of Stabilyser enables isolation of functionally active proteins, while maintaining integrity of nucleic acids. Stabilization of proteins and RNA is achieved even after 24 hours at room temperature Stabilyser reagent is compatible with Western blotting protocols, functional protein activity assays, and nucleic acid applications like qPCR.

Use in conjunction with a homognenizer for simultaneous tissue lysis and stabilization of protein and nucleic acids in tissue samples.

Application

Prevents degradation of proteins and nucleic acid during tissue lysis and storage. Stabilize functional proteins and RNA.

Features and Benefits

  • Gentle, non-denaturing lysis buffer does not interfere with downstream applications
  • Convenient ready-to-use reagent
  • Stabilizes RNA and protein for analysis at a later date

Preparation Note

Ready-to-use reagent. In general it is recommended to start optimization with 20 μL of Stabilyser reagent per mg of tissue.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Contenido relacionado

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An overview of cell lysis and protein extraction methods including detergent solubilization, freeze-thaw lysis, osmotic shock, sonication, enzymatic cell lysis, and mechanical disruption techniques such as Dounce, Polytron, and mortar and pestle homogenization.

Lysis & Protein Extraction

Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.

Lisis y extracción de proteínas

Visión general de los métodos de lisis celular y extracción de proteínas: solubilización con detergente, lisis mediante congelación-descongelación, choque osmótico, tratamiento con ultrasonidos, lisis celular enzimática y técnicas de alteración mecánica como la homogeneización con Dounce, Polytron y con mortero y almirez.

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