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Merck

PKH26PCL

Sigma-Aldrich

PKH26 Red Fluorescent Cell Linker Kit for Phagocytic Cell Labeling

Distributed for Phanos Technologies

Sinónimos:

Phagocytic cell label

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1 KIT
MXP 20,902.00

MXP 20,902.00


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1 KIT
MXP 20,902.00

About This Item

Código UNSPSC:
12352207
NACRES:
NA.32

MXP 20,902.00


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Aplicación

This kit is for phagocytic cell labeling. It is used to selectively label cells with phagocytic capabilities such as monocytes, macrophages or neutrophils.

Principio

The labeling occurs through the formation of dye aggregates or particulates. The aggregate formation significantly inhibits the uptake of dye by non-phagocytic cells, such as lymphocytes, but facilitates dye uptake by phagocytic cells. Labeled cells appear patchy or spotted because the dye is localized in phagocytic compartments of the cells. The dye appears to be resistant to metabolic attack and has been found to remain with the cells for at least 21 days in vivo.
Labeling of phagocytic cells by this methodology may be conducted either in vitro or in vivo. Intraperitoneal or intravenous injections of the PKH26 labeling solution will successfully label phagocytic cells in vivo, while cells of interest which have been isolated may be stained using in vitro labeling methods.

Ligadura / enlace

For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.

Información legal

CellVue is a registered trademark of Phanos Technologies

Solo componentes del kit

Referencia del producto
Descripción

  • Diluent B 6 x 10

  • PKH26 cell linker in ethanol .5 mL

Producto relacionado

Referencia del producto
Descripción
Precios

Pictogramas

FlameExclamation mark

Palabra de señalización

Danger

Frases de peligro

Clasificaciones de peligro

Eye Irrit. 2 - Flam. Liq. 2

Código de clase de almacenamiento

3 - Flammable liquids

Punto de inflamabilidad (°F)

57.2 °F - closed cup

Punto de inflamabilidad (°C)

14.0 °C - closed cup


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K Tabata et al.
Gene therapy, 18(10), 969-978 (2011-04-23)
We previously identified the mouse and human Glipr1 and GLIPR1/RTVP-1 genes, respectively, as direct p53 targets with proapoptotic activities in various cancer cell lines, including prostate cancer (PCa). Intratumoral injection of an adenoviral vector capable of efficient transduction and expression
Hyung-Syup Kim et al.
Journal of Korean Neurosurgical Society, 44(4), 249-255 (2008-12-20)
In Moyamoya disease, the primary goal of treatment is to improve collateral circulation through angiogenesis. In the present study, we obtained and sub-cultured bone marrow stromal cells (BMSCs) from rats without a cell-mediated immune response. Then, we injected the labeled
Carey N Lumeng et al.
Diabetes, 56(1), 16-23 (2006-12-29)
Although recent studies show that adipose tissue macrophages (ATMs) participate in the inflammatory changes in obesity and contribute to insulin resistance, the properties of these cells are not well understood. We hypothesized that ATMs recruited to adipose tissue during a
Radhika Thokala et al.
PloS one, 11(8), e0159477-e0159477 (2016-08-23)
Adoptive immunotherapy infusing T cells with engineered specificity for CD19 expressed on B- cell malignancies is generating enthusiasm to extend this approach to other hematological malignancies, such as acute myelogenous leukemia (AML). CD123, or interleukin 3 receptor alpha, is overexpressed
Joseph Park et al.
Frontiers in immunology, 10, 2231-2231 (2019-10-16)
Neuroinflammatory roles of central innate immunity in brain parenchyma are well-regarded in the progression of neurodegenerative disorders including Alzheimer's disease (AD), however, the roles of peripheral immunity in central nervous system (CNS) diseases are less clear. Here, we created a

Artículos

PKH dyes are easy to use and achieve stable, uniform, and reproducible fluorescent labeling of live cells. PKH dyes are non-toxic membrane stains which produce high signal to noise ratio.

Lipophilic cell tracking dyes enable cancer biologists to track tumor and immune cell functions both in vitro and in vivo. Read the article to choose a right membrane dye kit for cell tracking and proliferation monitoring.

Optimal staining is a key component for studying tumorigenesis and progression. Learn useful tips and techniques for dye applications, including examples from recent studies.

PKH and CellVue® Fluorescent Cell Linker Kits provide fluorescent labeling of live cells over an extended period of time, with no apparent toxic effects.

Questions

  1. Is this kit suitable for label circulatory fibrocyte isolated from rat PBMC?

    1 answer
    1. The PKH cell linker kits have not been specifically tested for the detection of circulatory fibrocytes. However, there are several publications that indicate suitability. Please see the link below to review one such reference:
      https://onlinelibrary.wiley.com/doi/full/10.1111/jcmm.13080

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