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Merck
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PF0100

Sigma-Aldrich

Protease Fluorescent Detection Kit

high sensitivity assay

Sinónimos:

Protease Assay Kit

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.26

shipped in

dry ice

storage temp.

−20°C

Application

Fluorescent Protease Detection Kit Video Protocol
Protease Fluorescent Detection Kit has been used:
  • in the detection of proteolytic activity using casein based protease assay
  • for testing the extracellular proteolytic activity of fungal strains
  • for quantifying mitochondrial protease activity
  • for measuring the protease production from soil solution

Suitability

Detection limit: approx. 50 ng trypsin

Quantity

Supplied with sufficient reagents for up to 200 one mL assays.
Supplied with sufficient reagents for up to 200 one mL assays.

The trichloroacetic acid (TCA) component is provided as 6.1 N TCA solution. 0.6 N TCA Solutions may be prepared by a 10-fold dilution of the desired volume of T0699 with ultrapure water. It is not necessary to dilute the entire volume of T0699 for immediate use of the kit.

Analysis Note

Tested for detection of all four proteolytic classes (serine, apartic, cysteine and metalloproteinases).

Solo componentes del kit

Referencia del producto
Descripción
  • Incubation buffer 25 mL
  • FITC-casein solution 5 mL
  • Assay buffer 200 mL
  • Protease standard (trypsin) 20 μg
  • FITC control 50 mg
  • Trichloroacetic acid (TCA) solution 30 mL

signalword

Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3

target_organs

Respiratory system

Storage Class

8A - Combustible corrosive hazardous materials

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Dramatic increase in stability and longevity of enzymes attached to monodispersive iron nanoparticles.
Sharma A, et al.
IEEE Transactions on Magnetics, 43, 2418-2420 (2007)
Joseph S Rom et al.
Virulence, 12(1), 584-600 (2021-02-05)
Using the USA300, methicillin-resistant Staphylococcus aureus strain LAC, we previously examined the impact of regulatory mutations implicated in biofilm formation on protease production and virulence in a murine sepsis model. Here we examined the impact of these mutations in the
Veronica Tjomsland et al.
European journal of immunology, 43(6), 1470-1483 (2013-03-26)
Induction of optimal HIV-1-specific T-cell responses, which can contribute to controlling viral infection in vivo, depends on antigen processing and presentation processes occurring in DCs. Opsonization can influence the routing of antigen processing and pathways used for presentation. We studied
Stimulation of soil organic nitrogen pool: The effect of plant and soil organic matter degrading enzymes
Kieloaho AJ, et al.
Soil Biology and Biochemistry, 96(10), 97-106 (2016)
Céline Adam et al.
PloS one, 7(5), e38138-e38138 (2012-06-14)
Mitochondria have their own ATP-dependent proteases that maintain the functional state of the organelle. All multicellular eukaryotes, including filamentous fungi, possess the same set of mitochondrial proteases, unlike in unicellular yeasts, where ClpXP, one of the two matricial proteases, is
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Protocolos

Protease Fluorescent Detection Kit Usage

Protease Fluorescent Detection Kit detects protease activity using FITC-labeled casein substrate.

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