P5899

Poly-ʟ-Lysine Hydrobromide

synthetic, mol wt ≥300,000, powder, γ-irradiated, suitable for cell culture, BioXtra

• Sinónimos: PDL HBr
• Número de CAS: 25988-63-0
• MDL number: MFCD00237230
• UNSPSC Code: 12352202
• eCl@ss: 32160406
• PubChem Substance ID: 24898718
• NACRES: NA.75

Propiedades

• Nombre del producto: Poli-L-lisina hydrobromide, mol wt ≥300,000, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture
• biological source: synthetic (organic)
• Quality Level: 200
• sterility: γ-irradiated
• product line: BioXtra
• form: lyophilized powder
• mol wt: ≥300,000
• packaging: pkg of 5 mg
• technique(s): cell culture | mammalian: suitable
• surface coverage: 4 μg/cm²
• impurities: <10% water (by Karl Fischer)
• solubility: H₂O: soluble 50 mg/mL, clear to very slightly hazy, colorless to faintly yellow
• shipped in: ambient
• storage temp.: −20°C
• SMILES string: Cl.NCCCCC(N)C(O)=O
• InChI: 1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1
• InChI key: WBSCNDJQPKSPII-KKUMJFAQSA-N

Descripción

Application

Poly-L-lysine hydrobromide has been used to coat:

• 2-well plates before seeding muscle cells in a research study on rainbow trout myotube formation[1]
• flat bottom 96-well plates for in vitro neuronal stem cell differentiation[2]
• T75 flasks for primary astrocytic cultures[3]

Biochem/physiol Actions

Poly-L-lysine serves as a coating substance to ensure better attachment of cells to culture surfaces.[4] Research shows that plates coated with poly-L-lysine (PLL) demonstrated an accelerated cell growth rate.[5] Culturing mesenchymal stem cells (MSCs) on PLL-coated substrates has been found to safeguard the stemness properties of these cells and inhibit senescence. PLL-coated plates have demonstrated enhanced MSC growth and up-regulated the expression of genes associated with cell adhesion, differentiation, proliferation, and signaling. Moreover, porous PLL-coated surfaces have shown improvements in cell proliferation and the osteogenic differentiation of MSCs.[5]

Components

La poli-L-lisina es una polímero de aminoácido con carga positiva con aproximadamente un HBr por residuo de lisina. El hidrobromuro permite a la poli-L-lisina estar en una forma cristalina hidrosoluble. Puede encontrarse una pequeña cantidad del producto en la estructura beta porque el HBr interfiere en el enlace de hidrógeno entre el grupo amino y los grupos carboxilo o los grupos que contienen N u O.

Caution

Las disoluciones estériles son estables durante un máximo de 2 años cuando se conservan entre 2 y 8°C. Deben guardarse desecadas a -20°C.

Analysis Note

Molecular weight based on viscosity. Also assayed by MALLS.

Other Notes

For additional technical information on polyamino acids please visit the Polyamino acid FAQ resource.

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)