P5622

Anti-Pen-2 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Número MDL: MFCD07370427
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• Formulario: buffered aqueous solution
• mol peso: antigen 10 kDa
• reactividad de especies: human
• técnicas: microarray: suitable; western blot: 3-6 μg/mL using whole extract of human kidney 293 cells expressing human Pen-2
• Nº de acceso UniProt: P49810
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... PSENEN(55851) ; mouse ... Psen2(19165)

Descripción

Descripción general

Presenilin enhancer γ-secretase subunit (Pen-2/ PSENEN) gene, with four exons spanning 1.4 kb, is mapped to human chromosome 19q13.12. Pen-2 is an integral membrane protein with 101 amino acids. The protein is characterized with two transmembrane domains and a cytosolic loop domain, with the C- and N-termini facing the lumen of the endoplasmic reticulum.

Especificidad

Anti-PEN2 antibody recognizes Pen-2 (10 kDa).

Inmunógeno

synthetic peptide encoding amino acids 86-101 located at the C-terminus of human pen-2, conjugated to KLH. This sequence is identical in mouse pen-2.

Aplicación

Anti-Pen-2 antibody produced in rabbit has been used in immunoblotting.

Acciones bioquímicas o fisiológicas

Pen-2 is a critical component of the presenilin (PS)1/γ-secretase and PS2/γsecretase complexes. siRNA interference mediated down regulation of pen-2 is due to reduced PS levels, impaired nicastrin (Nct) maturation and deficient γ secretase complex formation. Pen-2 preferentially interacts with PS1 and induce PS1 endoproteolysis. In addition, it also stabilizes PS fragment heterodimer within the γ-secretase complex subsequent to PS endoproteolysis. PEN-2 plays a vital role in γ-secretase complex maturation in the absence of PS endoproteolysis. Mutation in the PEN-2 gene leads to the development of familial comedones syndrome.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Almacenamiento y estabilidad

For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable