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Merck

P4109

Sigma-Aldrich

β-Phosphoglucomutase from Lactococcus sp.

recombinant, expressed in E. coli

Sinónimos:

β-D-Glucose 1,6-phosphomutase

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250 UNITS
MXP 3,240.00
1000 UNITS
MXP 8,495.00

MXP 3,240.00


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Seleccione un Tamaño

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250 UNITS
MXP 3,240.00
1000 UNITS
MXP 8,495.00

About This Item

Comisión internacional de enzimas:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54

MXP 3,240.00


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recombinante

expressed in E. coli

Formulario

solid

actividad específica

≥10 unit/mg solid

temp. de almacenamiento

−20°C

Aplicación

β-Phosphoglucomutase is used to study starch and sucrose metabolism[1]. It is used for the determination of α-amylase in clinical analysis. It is used to study galactose utilization[2] and generalized glycogenosis (Pompe′s Disease)[3].

Acciones bioquímicas o fisiológicas

β-Phosphoglucomutase enzymatically converts β-D-glucose 1-phosphate to β-D-glucose 6-phosphate. It is involved in starch and sucrose metabolism[1].
Enzymatically converts β-D-glucose-1-phosphate to β-D-glucose-6-phosphate.

Definición de unidad

One unit is defined as the amount of enzyme which converts 1μmol of β-D-glucose-1-phosphate to β-D-glucose-6-phosphate per minute at 37°C and pH 7.0.

Otras notas

Contains EDTA and lactose.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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F Levander et al.
Applied and environmental microbiology, 67(6), 2734-2738 (2001-05-26)
To study the influence of phosphoglucomutase (PGM) activity on exopolysaccharide (EPS) synthesis in glucose- and lactose-growing Streptococcus thermophilus, a knockout PGM mutant and a strain with elevated PGM activity were constructed. The pgmA gene, encoding PGM in S. thermophilus LY03
Brigitta Elsässer et al.
Journal of molecular modeling, 18(7), 3169-3179 (2012-01-13)
Enzyme catalyzed phosphate transfer is a part of almost all metabolic processes. Such reactions are of central importance for the energy balance in all organisms and play important roles in cellular control at all levels. Mutases transfer a phosphoryl group
Cecelia A Garcia et al.
Applied microbiology and biotechnology, 105(10), 4033-4052 (2021-05-08)
In a competitive microbial environment, nutrient acquisition is a major contributor to the survival of any individual bacterial species, and the ability to access uncommon energy sources can provide a fitness advantage. One set of soluble carbohydrates that have attracted
OCULAR HISTOPATHOLOGY IN GENERALIZED GLYCOGENOSIS (POMEPE'S DISEASE).
D TOUSSAINT et al.
Archives of ophthalmology (Chicago, Ill. : 1960), 73, 342-349 (1965-03-01)
A phosphoglucomutase specific for β-glucose 1-phosphate
Ben-Zvi R and Schramm M
The Journal of Biological Chemistry, 236, 2186-2189 (1961)

Questions

  1. Hello, I received the enzyme pgmB (P4109) and wondered in what buffer should I dissolve the enzyme powder. Thank you

    1 answer
    1. This product may be reconstituted in 10 mM Potassium Phosphate, pH 7 at 5mg/mL. The stock solution is expected to be stabe for at least 1 week when stored refrigerated. For assay purposes, the enzyme is prepared just prior to use with ice cold 10 mM Potassium Phosphate, pH 7 solution duluted to 1.0 - 3.0 U/mL.

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