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P4056

Sigma-Aldrich

Platelet-Derived Growth Factor-BB from rat

PDGF-BB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Sinónimos:

PDGF-BB

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About This Item

MDL number:
MFCD01094933
UNSPSC Code:
12352202
NACRES:
NA.77

recombinant

expressed in E. coli

Quality Level

100

form

lyophilized powder

potency

1.62 ng/mL EC50

quality

endotoxin tested

mol wt

~30 kDa

packaging

pkg of 5x50 μg
pkg of 50 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤1 EU/μg

UniProt accession no.

P31240

storage temp.

−20°C

Gene Information

rat ... Pdgfb(24628)

Categorías relacionadas

General description

Platelet-derived growth factor (PDGF) is a 30 kDa cationic polypeptide and consists of two disulphide linked chains A and B. PDGF is expressed as hetero and homodimers : PDGF-AB FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD.

Application

Platelet-Derived Growth Factor-BB from rat has been used as a growth supplement in mouse embryonic fibroblast culture, motoneuron-muscle co-culture and adult spinal cord neurons culture.

Biochem/physiol Actions

Platelet-derived growth factor (PDGF) elicits multifunctional actions with a variety of cells. It is mitogenic to mesoderm-derived cells, such as dermal and tendon fibroblasts, vascular smooth muscle cells, glial cells, and chondrocytes. PDGF is a potent chemoattractant and activator of neutrophils, monocytes, and fibroblasts. PDGF activates smooth muscle relocalization and multiplication. PDGF mediated increased smooth muscle muscle proliferation might be associated with gene regulation. It increases the synthesis of phospholipids, cholesterol esters, glycogen and prostaglandins, and modulates LDL (low density lipoprotein) receptor binding.

Other Notes

Dimer of the B chain of rat PDGF.

Physical form

Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 2.5 mg bovine serum albumin.

Analysis Note

The biological activity is measured by its ability to stimulate 3H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Platelet-Derived Growth Factor?BB Transactivates the Fibroblast Growth Factor Receptor to Induce Proliferation in Human Smooth Muscle Cells.
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Trends in Cardiovascular Medicine, 16.1, 25-28 (2006)
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Artículos

Platelet Derived Growth Factors (PDGF)

Platelet-Derived Growth Factor is the principal mitogen found in mammalian serum and is released from platelets during clot formation.

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