The product does not have a quality specification for pH and, consequently, is not tested.
P2287
TWEEN® 20
viscous liquid, suitable for cell culture
Sinónimos:
Monolaurato de sorbitán polietilenglicol, Monolaurato de sorbitán polioxietileno
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100 ML
MXP 967.00
500 ML
MXP 1,685.00
4 L
MXP 5,231.00
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100 ML
MXP 967.00
500 ML
MXP 1,685.00
4 L
MXP 5,231.00
About This Item
Productos recomendados
descripción
non-ionic
Formulario
viscous liquid
mol peso
~1228
composición
lauric acid, ≥40% (balance primarily myristic, palmoyic, and stearic acids)
concentración
≥40.0%
técnicas
cell culture | mammalian: suitable
índice de refracción
n20/D 1.468 (lit.)
CMC
0.06 mM (20-25°C)
temperatura de transición
cloud point 76 °C
solubilidad
water: soluble
densidad
1.095 g/mL at 25 °C (lit.)
1.1 g/cm3 at 25 °C (lit.)
HLB
16.7
cadena SMILES
CCCCCCCCCCCC(=O)OCCOCC(C1C(C(CO1)OCCO)OCCO)OCCO
InChI
1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3
Clave InChI
HMFKFHLTUCJZJO-UHFFFAOYSA-N
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Descripción general
Tween 20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.[1]
Aplicación
Detergente no iónico
TWEEN 20 has been used in staining buffer for in situ hybridization.[2] It has also been used to prevent non-specific adsorption of proteins to coverslip during sample preparation for FRET (Förster resonance energy transfer) experiments.[3]
TWEEN 20 is a nonionic detergent widely used in biochemical applications. It has been used as an emulsifying agent for the preparation of stable oil-in-water emulsions. TWEEN 20 has been used in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins).
TWEEN 20 has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. TWEEN 20 can be used for lysing mammalian cells at a concentration of 0.005 to 0.5%.
TWEEN 20 has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. TWEEN 20 can be used for lysing mammalian cells at a concentration of 0.005 to 0.5%.
Precaución
TWEEN 20 may not be stable to autoclaving, particularly with metal cations in buffer solutions. Autoclaving is not recommended without testing for changes in properties. TWEEN 20 is heat sensitive and will darken when exposed to elevated temperatures. Polysorbates have been reported to be incompatible with alkalis, heavy metal salts, phenols, and tannic acid. They may also reduce the activity of many preservatives. Aqueous solutions of polysorbates undergo autoxidation during storage, with changes being catalyzed by light, increased temperature, and copper sulfate.
Nota de preparación
TWEEN 20 is miscible in water (100mg/mL), yielding a clear, yellow solution. It is also miscible with alcohol, dioxane, and ethyl acetate. TWEEN 20 is insoluble in liquid paraffin and fixed oils.
Información legal
TWEEN is a registered trademark of Croda International PLC
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
527.0 °F - Pensky-Martens closed cup
Punto de inflamabilidad (°C)
275 °C - Pensky-Martens closed cup
Equipo de protección personal
Eyeshields, Gloves
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Have you ever tested the pH of TWEEN 20?
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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