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P0652

Protease from Streptomyces sp.

Name: Protease from <I>Streptomyces</I> sp.
Brand: SIGMA

Type XXI, ≥15 units/mg solid

Sinónimos:

actinase E, pronase E, Actinase E, Pronase E

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Número CAS:

9036-06-0

UNSPSC Code:

12352204

EC Number:

232-909-5

NACRES:

NA.54

MDL number:

MFCD00132092

eCl@ss:

32160410

Specific activity:

≥15 units/mg solid

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Propiedades

type

Type XXI

Quality Segment

200

form

powder

specific activity

≥15 units/mg solid

mol wt

~50 kDa

storage temp.

−20°C

Descripción

General description

Isoelectric point : 8.7
Inhibitors : Diisopropyl fluorophosphate, EDTA
Optimum pH : >=12
Optimum temperature : 60^oC
pH Stability : pH 5.0 - 11.5 (25^oC,24hr)
Thermal stability : below 50^oC (pH 8.3, 15min)

Application

Pronase E can be used to degrade antheraea pernyi silk fibroin films.

This enzyme is useful for proteolysis of insoluble protein and for structure investigation of protein. Proteases from Streptomyces griseus and Streptomyces omiyaensis have been used in a study to identify residue 71 as a crucial residue for differences in topological specificities. Proteases have also been used in a study to investigate production of protease as a by-product of streptomycin production.

A mixture of at least three proteolytic activities including an extracellular serine protease. In general, serine proteases display a wide range of substrate specificities, which are believed to be mediated by an active site composed of one Asp, one His, and a Ser residue in the molecule. This enzyme prefers to hydrolyze peptide bonds on the carboxyl side of glutamic or aspartic acid.

Completely inactivated by heating above 80 °C for 15-20 minutes.

Protease is typically used in nucleic acid isolation procedures in incubations of 0.5-3.0 hours supplemented with 0.2% sodium dodecyl sulfate and 10 mM EDTA.

Biochem/physiol Actions

Alkaline protease that is approximately twice as active at pH 11.0 than at the usual assay conditions for protease, pH 7.5 and 37 °C. By comparison, P5147 Type XIV protease is only approximately 25% as active at pH 11.0, 30 °C.

Physical form

This enzyme is more active at a higher pH range than the known alkaline protease, showing the proteolytic activity even in 0.2N NaOH solution. This enzyme is useful for proteolysis of insoluble protein and for structure investigation of protein.

Preparation Note

Collected from culture broth of S.Sp

Other Notes

One unit will hydrolyze casein to produce peptide equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 11.0 at 30 °C.

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Este artículo	P5147	P8811	C0794
Sigma-Aldrich P0652 Protease from Streptomyces sp.	Sigma-Aldrich P5147 Proteasa from Streptomyces griseus	Sigma-Aldrich P8811 Proteasa from Streptomyces griseus	Sigma-Aldrich C0794 Chitosanase from Streptomyces sp.
description Type XXI, ≥15 units/mg solid	description Type XIV, ≥3.5 units/mg solid, powder	description powder, BioReagent, suitable for mouse embryo cell culture, ≥3.5 units/mg solid	description buffered aqueous glycerol solution, ≥15 units/mg protein (E^1%)
specific activity ≥15 units/mg solid	specific activity ≥3.5 units/mg solid	specific activity ≥3.5 units/mg solid	specific activity ≥15 units/mg protein (E^1%)
form powder	form powder	form powder	form buffered aqueous glycerol solution
mol wt ~50 kDa	mol wt -	mol wt -	mol wt -
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
type Type XXI	type Type XIV	type -	type -