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N3013

Sigma-Aldrich

Nile Red

Powder

Sinónimos:

Nile Blue A Oxazone

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100 MG
MXP 3,569.00
1 G
MXP 19,130.00

MXP 3,569.00

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100 MG
MXP 3,569.00
1 G
MXP 19,130.00

About This Item

Fórmula empírica (notación de Hill):
C20H18N2O2
Número de CAS:
7385-67-3
Peso molecular:
318.37
Beilstein:
279110
Número CE:
230-966-0
Número MDL:
MFCD00011639
Código UNSPSC:
12171500
ID de la sustancia en PubChem:
24897612
NACRES:
NA.47

MXP 3,569.00

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Nombre del producto

Nile Red, Technical grade

grado

technical grade

Formulario

powder

composición

Carbon: 70.0-77.3%

Nitrogen: 7.5-8.8%

técnicas

titration: suitable

color

faint green to brown

mp

203-205 °C (lit.)

solubilidad

methanol: 1 mg/mL

λmáx.

553 nm

aplicaciones

diagnostic assay manufacturing
hematology
histology

temp. de almacenamiento

room temp

cadena SMILES

CCN(CC)c1ccc2N=C3C(Oc2c1)=CC(=O)c4ccccc34

InChI

1S/C20H18N2O2/c1-3-22(4-2)13-9-10-16-18(11-13)24-19-12-17(23)14-7-5-6-8-15(14)20(19)21-16/h5-12H,3-4H2,1-2H3

Clave InChI

VOFUROIFQGPCGE-UHFFFAOYSA-N

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Descripción general

Nile Red is a lipophilic fluorescent dye that is commonly used in various scientific applications, particularly in lipid research. It has the ability to stain and visualize lipid droplets in cells and tissues.

Aplicación

Nile red is widely employed in studies related to lipid metabolism, lipid droplet dynamics, lipid accumulation in cells or tissues, and lipid-based drug delivery systems.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number
SLBP9326V
070K3462
063K3730
063K3691
061K3444

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Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Zebrafish obesogenic test: a tool for screening molecules that target adiposity.
Tingaud-Sequeira A
Journal of Lipid Research, 52, 1765-1765 (2011)
Autophagy gene disruption reveals a non-vacuolar cell death pathway in Dictyostelium.
Kosta A
The Journal of Biological Chemistry, 279, 48404-48404 (2004)
D L Sackett et al.
Analytical biochemistry, 167(2), 228-234 (1987-12-01)
Nile red is an uncharged hydrophobic molecule whose fluorescence is strongly influenced by the polarity of its environment. It interacts with many, but not all, native proteins, including beta-lactoglobulin, kappa-casein, and albumin, with a wide range of spectral changes for
Pei-Ying Chen et al.
Plant physiology, 180(2), 813-826 (2019-03-23)
Mitochondrial fission occurs frequently in plant cells, but its biological significance is poorly understood because mutants specifically impaired in mitochondrial fission do not show obvious defects in vegetative growth. Here, we revealed that the production of viable pollen was reduced
Glycine N-methyltransferase deficiency affects Niemann-Pick type C2 protein stability and regulates hepatic cholesterol homeostasis.
Liao YJ
Molecular Medicine, 18, 412-412 (2012)
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Questions

  1. How should n3013 be dissolved and diluted for lipid staining?

    1 answer

    1. There has not been any conduct suitability testing for Nile Red. Please see the following for an article that may offer information; J. Histochem. Cytochem. 35:619-621 reference.
      To perform the staining, a stock solution of Nile red was prepared by dissolving 10 mg of Nile red in 10 ml of acetone, which was then stored in the refrigerator protected from light. The sections were fixed in 1% glutaraldehyde in PBS for 5 minutes and washed in PBS three times for 2 minutes each. The staining solution was then prepared by diluting 100 ul of the stock solution in 1 ml of PBS. The sections were covered with this solution for 20 minutes at room temperature, then briefly washed in PBS and mounted in 75% glycerol in PBS. Other sections were incubated with solutions containing 50, 10, and 1 ug and 100 ng of Nile red per ml. The sections were examined with a filter set for fluorescein fluorescence (450-500 nm band pass excitation filter, a 510 nm center wavelength chromatic beam splitter, and a 528 nm long-pass barrier filter) and were photographed.

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