Saltar al contenido
Merck
Todas las fotos(3)

Documentos clave

MAK044

Sigma-Aldrich

kit de cuantificación de ácidos grasos libres

sufficient for 100 colorimetric or fluorometric tests

Sinónimos:

Kit de ensayo de ácidos grasos libres

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(3)

About This Item

Código UNSPSC:
12161503

uso

sufficient for 100 colorimetric or fluorometric tests

aplicaciones

cosmetics
food and beverages

método de detección

colorimetric
fluorometric

enfermedades relevantes

endocrinological disorders, diabetes; cancer

temp. de almacenamiento

−20°C

Descripción general

Los ácidos grasos libres, también conocidos como ácidos grasos no esterificados, circulan en el plasma unidos a la albúmina. La mayoría de los ácidos grasos libres o bien proceden de fuentes alimenticias o bien se movilizan desde el tejido adiposo. Los ácidos grasos libres en el plasma están elevados en muchas enfermedades relacionadas con la obesidad y pueden contribuir a la resistencia a la insulina en los tejidos periféricos. Enfermedades como la septicemia y los tumores que producen hormonas lipoactivas también pueden estar asociadas con concentraciones elevadas de ácidos grasos libres.

Las anomalías en el metabolismo de los ácidos grasos producen la enfermedad osteatosis hepática.[1] La acumulación de ácidos grasos libres en los miocitos provoca el riesgo de disfunción cardiaca.[2] El aumento de la β-oxidación de los ácidos grasos está implicado en la isquemia cardiaca.[2]

Aplicación

El kit de cuantificación de los ácidos grasos libres se ha utilizado en lo siguiente:
  • medir la hidrólisis lipídica mediante la determinación de los ácidos grasos libres en la muestra digerida.[3]
  • medir la concentración de ácidos grasos libres en plasma y suero.[4][5]
  • cuantificar la concentración de ácidos grasos libres en el tejido hepático.[6]

Idoneidad

Adecuado para detectar ácidos grasos libres de cadena larga en varias muestras biológicas, como el suero y el plasma, y otros líquidos del organismo, el alimento, los medios de crecimiento, etc.

Principio

En este kit, la concentración de ácidos grasos (C8 y más largos) se determina por un análisis enzimático acoplado, que da lugar a un producto colorimétrico (570 nm)/fluorométrico (λex = 535/λem = 587 nm), proporcional al ácido graso presente.

Producto relacionado

Referencia del producto
Descripción
Precios

92210

Timestrip Plus™ 0 °C

06693

Timestrip Plus™ -20 °C

sustituido por

Referencia del producto
Descripción
Precios

Pictogramas

Health hazard
GHS08

Palabra de señalización

Danger

Frases de peligro

H334

Consejos de prudencia

P261 - P284 - P501

Clasificaciones de peligro

Resp. Sens. 1

Código de clase de almacenamiento

10 - Combustible liquids

Punto de inflamabilidad (°F)

188.6 °F - closed cup

Punto de inflamabilidad (°C)

87 °C - closed cup

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number
8L11K06120
8A17K06120
8C21K06120
8E18K06120
8B15K06120

¿No ve la versión correcta?

Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

Busque por un COA

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Interactions among macronutrients in wheat flour determine their enzymic susceptibility
Bhattarai R R, et al.
Food Hydrocolloids, 61, 415-425 (2016)
Estrogen Receptor Involvement in Noradrenergic Regulation of Ventromedial Hypothalamic Nucleus Glucoregulatory Neurotransmitter and Stimulus-Specific Glycogen Phosphorylase Enzyme Isoform Expression.
A S M H Mahmood et al.
ASN neuro, 12, 1759091420910933-1759091420910933 (2020-04-03)
Changes in feed intake during isolation stress in respiration chambers may impact methane emissions assessment.
Llonch P, et al.
Animal Production Science, 58(6), 1011-1016 (2018)
Association of Temperament and acute stress responsiveness with Productivity, Feed efficiency, and Methane emissions in Beef cattle: an Observational study
Llonch P, et al.
Frontiers in veterinary science, 3, 43-43 (2016)
Sarmistha Mukherjee et al.
Hepatology (Baltimore, Md.), 65(2), 616-630 (2016-11-04)
The regenerative capacity of the liver is essential for recovery from surgical resection or injuries induced by trauma or toxins. During liver regeneration, the concentration of nicotinamide adenine dinucleotide (NAD) falls, at least in part due to metabolic competition for
Ver todos los artículos relacionados

Questions

1–9 of 9 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Is there a step in the process where samples can be preserved for future analysis using the kit?

    1 answer

    1. Yes, samples can be preserved for future analysis using the kit by aliquoting and freezing them if needed for multiple uses. However, it's recommended to use fresh samples whenever possible to ensure accurate results. Dried lipids can be stored in 200 µl of Fatty Acid Assay Buffer at -80°C. In case of lipid precipitation, re-dissolve the lipids by sonication. However, the supplier does not specify the maximum duration for which the samples stored at -80°C will remain stable. Storing samples at -80°C may impact their stability, potentially resulting in lower than expected readings. Therefore, it is recommended to use fresh samples for this assay whenever possible to ensure accurate results.
      If frozen samples are used, they should be snap frozen in liquid nitrogen immediately after collection. These samples cannot undergo multiple freeze-thaw cycles and must be stored for relatively short periods. It's important to note that stability studies on different sample types have not been conducted, so there is no advice available regarding the timeframe for measuring frozen samples.

      Helpful?

  4. Can MAK044 quantify only albumin-bound free fatty acids (FFA), or does it also quantify unbound FFA?

    1 answer

    1. The kit specifically detects unbound free fatty acids in the samples and does not detect any albumin-bound free fatty acids.

      Helpful?

  5. What are the recommended storage instructions for the Enhancer reagent and unused Assay Buffer in the MAK044 Free Fatty Acid Quantitation Kit?

    1 answer

    1. The enhancer can be aliquoted and stored at -20°C, while the assay buffer can also be stored in aliquots at -20°C to avoid multiple freeze-thaw cycles.

      Helpful?

  6. I would like to quantify butyric acid in bacteria culture supernatant. Does this MAK044 Free Fatty Acid Quantitation Kit suitable or is there a kit you can recommend. Thank you. 

    1 answer

    1. Butyric acid is a short-chain fatty acid. This kit is suitable for detecting the long-chain free fatty acids in various biological samples, such as serum, plasma, and other body fluids, food, growth media, etc. The Short Chain Fatty Acid Kit, prod number SBR0030, offers a potential analytical option. This kit is suitable for detection of several SCFAs, including Butyric, by HPLC, LC-MS, and GC-MS. See the link below to the technical bulleting should these methods be suitable:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/593/831/SBR00030bul.pdf

      Helpful?

  7. Can this kit be used to analyse NEFA in plasma using the Pentra C400 instrument?

    1 answer

    1. This kit is suitable for detecting nonesterified fatty acids in plasma (NEFA). However, suitability with Pentra C400 instrument has not been determined. This application must be determined by the end-user.

      Helpful?

  8. Can these kits be used with serum samples from mouse?

    1 answer

    1. Yes, this kit can be used on mouse serum samples. We generally recommend that fresh serum samples be used. Frozen samples can probably be used. However, the successful use of frozen samples depends on the efficiency of freezing them and the amount of time they have been frozen. If previously frozen serum samples are used, you may want to check the performance of the kit with fresh vs. frozen samples.

      Helpful?

  9. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

Reviews

No rating value

Active Filters

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico