Saltar al contenido
Merck
Todas las fotos(1)

Documentos

M2154

Sigma-Aldrich

Medium 199

With Earle′s salts and sodium bicarbonate, without ʟ-glutamine, sterile liquid, suitable for cell culture

Sinónimos:

M199 Medium, TCM 199

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(1)

About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

product name

Medium 199, With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

pH

7.0-7.6 (25 °C, 209 g/L)

components

sodium pyruvate: no
L-glutamine: no
NaHCO3: yes
HEPES: no
phenol red: yes
Earle’s salts (5% CO2): yes

shipped in

ambient

storage temp.

2-8°C

¿Está buscando productos similares? Visita Guía de comparación de productos

Categorías relacionadas

General description

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Application

Medium 199 has been used:
  • to culture rumen epithelial cells from yearling Speckle Park beef heifers
  • as a component of the maturation medium for the incubation of Cumulus oocyte complexes (COCs) from bovine ovaries
  • as a component of the Dulbecco′s modified Eagle medium (DMEM) mixture to maintain rat neonatal cardiac myocytes (NCM)

Reconstitution

Supplement with 0.1 g/L L-glutamine.

also commonly purchased with this product

Referencia del producto
Descripción
Precios

supplement

Referencia del producto
Descripción
Precios

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

E Held-Hoelker et al.
Theriogenology, 96, 145-152 (2017-05-24)
Lipid accumulation is associated with reduced embryonic quality, causing limited survival after cryopreservation. Therefore, in the present study we aimed to reveal the effects of supplementation of a lipid reducing agent, l-carnitine and the removal of fatty acids during in vitro
C Kent-Dennis et al.
Journal of dairy science, 104(1), 1002-1017 (2020-11-03)
Inflammation of ruminal epithelium may occur during ruminal acidosis as a result of translocation and interaction of ruminal epithelial cells (REC) with molecules such as lipopolysaccharide (LPS). Such inflammation has been reported to alter cellular processes such as nutrient absorption
Alfred Nguyen et al.
Lab on a chip, 21(10), 1929-1947 (2021-05-20)
Active microrheology is one of the main methods to determine the mechanical properties of cells and tissue, and the modelling of these viscoelastic properties is under heavy debate with many competing approaches. Most experimental methods of active microrheology such as
Michelle M Halstead et al.
Nature communications, 11(1), 4654-4654 (2020-09-19)
The shift from maternal to embryonic control is a critical developmental milestone in preimplantation development. Widespread transcriptomic and epigenetic remodeling facilitate this transition from terminally differentiated gametes to totipotent blastomeres, but the identity of transcription factors (TF) and genomic elements
Nigel J Brand et al.
Methods in molecular biology (Clifton, N.J.), 633, 113-124 (2010-03-06)
We describe a method of isolating and maintaining primary cultures of mouse neonatal cardiac myocytes (NCM). This is derived from the well-established procedure for making NCM cultures from rat neonates by sequential digestion of rat ventricular myocardial pieces using a
Ver todos los artículos relacionados

Artículos

Medium 199 - M199 Formulations

Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico