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M0650

Medium 199

With Earle′s salts, without ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture, 10X

Sinónimos:

Cell growth medium, M199 Medium, TCM 199

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A ustedes/SKUDisponibilidadPrecio
100 mL
Comprobar disponibilidad del carrito
MXP 770.00

Acerca de este artículo

UNSPSC Code:
12352207
NACRES:
NA.75

MXP 770.00

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Nombre del producto

Medium 199, 10 ×, With Earle′s salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Quality Segment

400

sterility

sterile-filtered

form

liquid

concentration

10 ×

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: no
phenol red: yes
sodium pyruvate: no
HEPES: no
Earle’s salts (5% CO2): yes
L-glutamine: no

shipped in

ambient

storage temp.

2-8°C

General description

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Application

Medium 199 has been used:

  • to culture human mesothelial cells[1]
  • as a component of the holding medium for the collection of embryos from pluriparous Boer goat
  • in the preparation of spheroid-embedded collagen matrices[2]

Preparation Note

Supplement with 0.1 g/L L-glutamine, 2.2 g/L sodium bicarbonate at 1×.

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Este artículo
M9163M7528M5017
Medium 199 10 ×, With Earle′s salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

M0650

Medium 199

Medium 199 10 ×, With Hanks′ salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

M9163

Medium 199

Medium 199 HEPES Modification, with Earle′s salts, 25 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

M7528

Medium 199

Medium 199 With Earle′s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Sigma-Aldrich

M5017

Medium 199

form

liquid

form

liquid

form

liquid

form

powder

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

-

components

NaHCO3: no
phenol red: yes
sodium pyruvate: no
HEPES: no
Earle’s salts (5% CO2): yes
L-glutamine: no

components

phenol red: yes
HEPES: no
sodium pyruvate: no
Hanks’ salts (2% CO2): yes
L-glutamine: no
NaHCO3: no

components

L-glutamine: no
sodium pyruvate: no
HEPES: 25 mM
phenol red: yes
NaHCO3: yes
Earle’s salts (5% CO2): yes

components

L-glutamine: yes
phenol red: yes
NaHCO3: no
sodium pyruvate: no
Earle’s salts (5% CO2): yes
HEPES: no

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

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Clase de almacenamiento

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Questions

  1. If I have 10x Medium, how can I dilute it in order to have 1x and grow cells?

    1 answer

    1. Since certain components in 10X medium concentrates precipitate at pH 7.0, it is necessary to adjust the pH of these solutions to maintain solubility.
      Therefore, it may be necessary for the user to adjust the pH of the 1X preparation with sterile 1 N NaOH or 1 N HCl. Procedure For Dilution to 1X (1 Liter):
      (NOTE: Dilution of 10X solutions should be performed with sterile containers, components, and equipment.)
      • Aseptically measure out approximately 850 ml of tissue culture grade water into an appropriate size container.
      • While gently stirring the water, add 100 ml of 10X medium.
      • To the solution, add the required amount of sodium bicarbonate ( 0.35 g/L) and L-glutamine (0.1 g/L), at 1X
      • While stirring, adjust the solution to desired pH with 1 N NaOH or 1 N HCl.
      • Bring medium to final volume with additional tissue culture grade water.
      • It is recommended to sterile filter the prepared medium through a 0.22 ?m sterile membrane, or, for example, a Stericup.
      • Aseptically dispense into sterile containers. Store refrigerated at 2-8° C

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