L7886

Lectin from Ricinus communis (castor bean) Agglutinin RCA₁₂₀

buffered aqueous solution

• MDL number: MFCD00165777
• UNSPSC Code: 12352200
• NACRES: NA.32

Propiedades

• form: buffered aqueous solution
• potency: <4 μg/mL agglutination activity
• Storage temp.: 2-8°C

Descripción

General description

Lectins are carbohydrate binding proteins.[1] Lectin from Ricinus communis (castor bean) Agglutinin RCA₁₂₀ is purified from the seeds of the castor plant. It is a tetrameric hemagglutinin with two α and two β subunits.[2]

Application

Lectin from Ricinus communis (castor bean) Agglutinin RCA120 has been used for the digestion, enrichment and deglycosylation of chicken egg white (CEW) glycopeptides.[3] It has also been used as a model carbohydrate-binding protein.[4]

Biochem/physiol Actions

Ricinus communis agglutinin (RCA) occurs in two forms designated RCA₆₀ and RCA₁₂₀ according to their mol. wt. of approx. 60,000 and 120,000 respectively. Neither RCA₆₀ nor RCA₁₂₀ is blood group specific. RCA₆₀, also referred to as RCA_II, Ricin D or RCL III is extremely toxic, inhibits protein synthesis and has an affinity for N-acetyl-D-galactosamine. RCA₁₂₀, also referred to as RCA_I or RCL (I + II), is an agglutinin and has an affinity for terminal β-D-galactosyl residues.
Conjugates are prepared from affinity purified agglutinin RCA₁₂₀.

Lectin from Ricinus communis (castor bean) Agglutinin RCA₁₂₀ is used to study cell surface glycoproteins the separation of glycoproteins with Galβ-terminating oligosaccharide chains from glycolipids.[2] Lectin agglutinates cells and precipitates polysaccharides and glycoproteins.[1]

Physical form

Solution in 0.005 M sodium phosphate and 0.2 M sodium chloride, pH 7.2, containing 0.1% sodium azide

Preparation Note

Purified by affinity chromatography using the method of Lin and Li.[5]

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 7.2, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hour incubation at 25 °C.

Información de seguridad

• Storage Class: 10 - Combustible liquids
• wgk_germany: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)