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Merck

L4895

Sigma-Aldrich

Lectin from Triticum vulgaris (wheat)

FITC conjugate, lyophilized powder

Sinónimos:

WGA, Wheat germ agglutinin

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2 MG
MXP 3,377.00
5 MG
MXP 5,281.00
10 MG
MXP 9,726.00

MXP 3,377.00


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2 MG
MXP 3,377.00
5 MG
MXP 5,281.00
10 MG
MXP 9,726.00

About This Item

Código UNSPSC:
12352202
NACRES:
NA.32

MXP 3,377.00


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conjugado

FITC conjugate

Nivel de calidad

Formulario

lyophilized powder

potencia

<50 μg/mL agglutination activity

composición

Protein, ~50% Lowry

Extensión del etiquetado

~2 mol FITC per mol protein

temp. de almacenamiento

2-8°C

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Descripción general

Lectins are carbohydrate-binding proteins. They are omnipresent and usually found in fungi, plants and animals. The secondary structure of this protein is rich in β-strands and possesses carbohydrate binding sites on the surface.[1]

Aplicación

For the fluorescent detection of glycoproteins containing β(1→4)-N-acetyl-D-glucosamine
Lectin from Triticum vulgaris (wheat) has been used:
  • to treat transfected cells for immunofluorescence[2]
  • to stereotaxically inject into mice for neuronal tracing[3]
  • in immunohistochemistry for the analysis of myocytes[4]

Acciones bioquímicas o fisiológicas

Lectin plays a key role in glycoconjugate characterizing, imaging and targeting. Its use in a microarray assay, enable efficient glycome profiling. In plants and fungi, lectin defends against pathogens/feeders. Lectin also plays a major role in host recognition and tissue adhesion.[1]
WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.

Envase

Package size based on protein content.

Forma física

Contains phosphate buffer salts

Nota de preparación

Conjugates are prepared from affinity purified lectin.

Producto relacionado

Referencia del producto
Descripción
Precios

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)


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Rui Guo et al.
Scientific reports, 7(1), 8703-8703 (2017-08-20)
Diabetes is a major risk factor for cardiovascular disease and the lysosomal cysteine protease cathepsin K plays a critical role in cardiac pathophysiology. To expand upon our previous findings, we tested the hypothesis that, knockout of cathepsin K protects against
Robert H Oakley et al.
Science signaling, 12(577) (2019-04-18)
Stress is increasingly associated with heart dysfunction and is linked to higher mortality rates in patients with cardiometabolic disease. Glucocorticoids are primary stress hormones that regulate homeostasis through two nuclear receptors, the glucocorticoid receptor (GR) and mineralocorticoid receptor (MR), both
Novel Chemical Suppressors of Long QT Syndrome Identified by an In Vivo Functional Screen
Peal DS, et al.
Circulation (2011)
Yihua Bei et al.
Journal of cellular and molecular medicine, 21(8), 1648-1655 (2017-03-18)
The adult heart retains a limited ability to regenerate in response to injury. Although exercise can reduce cardiac ischaemia/reperfusion (I/R) injury, the relative contribution of cardiac cell proliferation including newly formed cardiomyocytes remains unclear. A 4-week swimming murine model was
Sarah Aileen Lewis et al.
PloS one, 13(8), e0201986-e0201986 (2018-08-14)
Tissues with high-energy demand including the heart are rich in the energy-producing organelles, mitochondria, and sensitive to mitochondrial dysfunction. While alterations in mitochondrial function are increasingly recognized in cardiovascular diseases, the molecular mechanisms through which changes in mitochondria lead to

Artículos

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

Understand sialic acid structure, function, signaling, and modifications. Easily find products for sialic acid research.

Questions

1–2 of 2 Questions  
  1. Please is this product use in staining Arbuscular mycorrhizal fungi in root, for confocal microscopy examination?

    1 answer
    1. This product has not been tested specifically for use in fungal staining in plant tissue. However, FITC-labeled wheat germ agglutinin or lectin has been cited in the literature for such applications. Please see the link below to review one such publication:
      https://iris.unito.it/bitstream/2318/1740844/1/Carotenuto%20et%20al%202.pdf

      Helpful?

  2. Does Sigma provide a stain to visualize cell borders for human Cardiomyocytes derived from the GM23338 iPSC cell line?

    1 answer
    1. There is no recommended stain for cells from this type of cell line. However, for visualizing typical cardiomyocyte cell borders, one may consider L4895, as referenced by Vanhoutte D et al, Nat Commun. 2021 Jun 24;12(1):3928. L4895 is Sigma-Aldrich Lectin from Triticum vulgaris (wheat). The article titled "Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy" in Nat Commun. 2021 Jun 24;12(1):3928 may serve as a point of reference.

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