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L4516

Sigma-Aldrich

Lipopolysaccharides from Escherichia coli O127:B8

BioXtra, suitable for cell culture, γ-irradiated

Sinónimos:

LPS

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1 MG
MXP 1,480.00

MXP 1,480.00

Disponible para envío el07 de abril de 2025Detalles

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1 MG
MXP 1,480.00

About This Item

Número de CAS:
93572-42-0
Número CE:
297-473-0
Número MDL:
MFCD00212998
Código UNSPSC:
12352211
NACRES:
NA.75

MXP 1,480.00

Disponible para envío el07 de abril de 2025Detalles

Solicitar un pedido a granel

origen biológico

Escherichia coli (O127:B8)

Nivel de calidad

200

esterilidad

γ-irradiated

Línea del producto

BioXtra

Formulario

lyophilized powder

purificado por

gel-filtration chromatography

técnicas

cell culture | mammalian: suitable

impurezas

<1% Protein (Lowry)

solubilidad

H2O: 5 mg/mL, slightly hazy

temp. de almacenamiento

2-8°C

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Categorías relacionadas

Aplicación

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).
Lipopolysaccharides from Escherichia coli 0127:B8 has been used:
  • to study the LPS-induced macrophage survival in mice[1]
  • to elicit the secretion of cytokines by cattle PBMC[2]
  • to study apoptosis of highly susceptible macrophage cells in a LPS-induced proinflammatory environment[1]
  • to perform autophagy assay in BV2 and BV2-LC3 cells[3]
  • for stimulation of human monocytes, macrophages and mouse microglia cells[4][5][6][7]
  • to induce pentraxin-3 expression in 293T cells[8]

Acciones bioquímicas o fisiológicas

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4). Being an immunogenic antigen, it enhances immune responses to soluble antigens.[9][10]

Reconstitución

Lipopolysaccharides are supplied as lyophilized, γ-irradiated powders. To reconstitute, add 1 ml sterile balanced salt solution or tissue culture medium to the vial (1 mg) and gently swirl until the powder dissolves. Reconstituted product may be further diluted to desired working concentrations using sterile balanced salt solution or tissue culture medium.

Otras notas

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Producto relacionado

Referencia del producto
Descripción
Precios

Pictogramas

Skull and crossbones
GHS06

Palabra de señalización

Danger

Frases de peligro

H300

Clasificaciones de peligro

Acute Tox. 2 Oral

Código de clase de almacenamiento

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000370464
0000370464
0000242816
0000242816
0000185322

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Li Wei et al.
Journal of neuroinflammation, 14(1), 198-198 (2017-10-05)
Several G-protein-coupled receptors (GPCRs) have been shown to be important signaling mediators between neurons and glia. In our previous screening for identification of nerve injury-associated GPCRs, G-protein-coupled receptor 84 (GPR84) mRNA showed the highest up-regulation by microglia after nerve injury.
Hua Liu et al.
International journal of clinical and experimental pathology, 6(4), 660-668 (2013-04-11)
The aim of the present study was to explore the role of receptor tyrosine kinases (RTKs) in the regulation of expression of PTX-3, a protector in atherosclerosis. Human monocytic U937 cells were infected with a shRNA lentiviral vector library targeting
Srinivas Akula et al.
Cells, 9(9) (2020-09-23)
Bone marrow-derived mast cells (BMMCs) are often used as a model system for studies of the role of MCs in health and disease. These cells are relatively easy to obtain from total bone marrow cells by culturing under the influence
Debby Kruijsen et al.
Vaccine, 29(15), 2730-2741 (2011-02-15)
Inactivated respiratory syncytial virus (RSV) vaccines tend to predispose for immune mediated enhanced disease, characterized by Th2 responses and airway hypersensitivity reactions. We show in a C57BL/6 mouse model that the early innate response elicited by the challenge virus (RSV
W A Jensen et al.
Journal of virology, 66(7), 4427-4433 (1992-07-01)
The in vitro expression of bovine leukemia virus (BLV) in short-term cultured bovine peripheral blood mononuclear cells (PBMC) is associated with increased spontaneous lymphocyte blastogenesis. The purpose of this study was to determine whether intracellular pathways responsible for antigen- or
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Artículos

Lipopolysaccharides for Research – Structure, Function & Applications

Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.

Questions

1–4 of 4 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. Once reconstituted what is the stability at -20C for the LPS product L4516-1M?

    1 answer

    1. Frozen aliquots can be stored up to 2 years. For more information, please see the link below:
      https://www.sigmaaldrich.com/technical-documents/protocol/clinical-testing-and-diagnostics-manufacturing/bacteriology/lipopolysaccharides

      Helpful?

  4. Can LPS bind to plastics (ex. 1.5 mL microtube) even when LPS solution is frozen stored at -20℃? I store LPS solution at 0.05mg/mL in -20℃ now.

    1 answer

    1. LSP is less likely to stick to plastics than glass, however very low concentrations (<0.1 mg/mL in water), LPS tends to stick to containers made
      of certain types of glass or plastic. To minimize, prepare stock solutions of at least 1 mg/mL. If the LPS concentration is >1 mg/mL, adsorption to the sides of the vial is
      negligible. For solutions stored in glass, vortexing for 30 minutes following thaw is recommended to redissolve. This may be useful for dilute solutions in plastic, as well. Surfaces (glass and plastics) may be salinized to reduce adherence.

      See Sigmacote, SL2:
      https://www.sigmaaldrich.com/product/sigma/sl2

      For additional information, see the link below to review the Lipopolysaccharides product information page:
      https://www.sigmaaldrich.com/technical-documents/protocol/clinical-testing-and-diagnostics-manufacturing/bacteriology/lipopolysaccharides

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