KEM0031

Klenow Fragment

Ultra-pure enzyme for nucleic acid modifications

Propiedades

• grade: for molecular biology
• assay: >99% (SDS-PAGE)
• form: buffered aqueous solution
• specific activity: 5,000 U/mg
• concentration: 5,000 U/mL
• shipped in: dry ice
• storage temp.: −20°C

Descripción

General description

Klenow Fragment is a mesophilic DNA polymerase derived from the E.coli Polymerase I DNA-dependent repair enzyme. The enzyme exhibits DNA synthesis and proofreading (3′ → 5′) nuclease activities, and, in the absence of the holoenzyme′s (5′→3′) nuclease domain, displays a moderate strand displacement activity during DNA synthesis. The protein is expressed as a truncated product of the E.coli PolA gene.

application

Suitable for:

• DNA blunting by fill-in of 5′ overhang
• Second strand cDNA synthesis
• Sequencing
• Site-specific mutagenesis

Features and Benefits

• Ultra-purification process for ultimate enzyme performance
• Highest quality specifications for ultimate product consistency
• Undetectable DNA and nuclease contamination

Components

Supplied with:KEM0042B (10X Blue Buffer)

Unit Definition

1 unit is defined as the amount of polymerase required to convert 10 nmol of dNTPs into acid insoluble material in 30 minutes at 37° C.

Physical form

Supplied in 100 mM KPO₄, 1.0 mM DTT, 0.1 mM EDTA, and 50% glycerol at pH 7.4 @ 25° C.

Other Notes

Source of protein: A recombinant E. coli strain carrying the Klenow Fragment gene.

Unit size: 2,500 U

Información de seguridad

• Storage Class: 10 - Combustible liquids