K4263

Anti-Kaiso antibody ,Mouse monoclonal

clone 6F, purified from hybridoma cell culture

• MDL number: MFCD04040940
• UNSPSC Code: 12352203

Propiedades

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: 6F, monoclonal
• form: buffered aqueous solution
• species reactivity: human, mouse, rat, canine, chicken
• technique(s): microarray: suitable; western blot: 1-2 μg/mL using CSMLO (mouse mammary adenocarcinoma) cell extracts
• isotype: IgG1
• UniProt accession no.: Q86T24
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ZBTB33(10009) ; mouse ... Zbtb33(56805) ; rat ... Zbtb33(501506)

Descripción

General description

Monoclonal Anti-Kaiso (mouse IgG1 isotype) is derived from the 6F hybridoma produced by the fusion of mouse myeloma cells and splenocytes from A/J mice. Kaiso is a DNA-binding protein and a member of the broad complex, tramtrak, bric a brac/ pox zinc finger (BTB/POZ) family of transcription factors with zinc finger motifs. This protein contains three carboxy-terminal zinc-finger motifs of the Kruppel-like C2H2-DNA binding type zinc fingers and a poxvirus and zinc finger (POZ) domain in its extreme N-terminus, which is highly hydrophobic. The zinc finger and BTB domain containing 33 (ZBTB33) gene is located on the human chromosome at Xq24.

Specificity

Monoclonal Anti-Kaiso reacts specifically with human, rat, mouse, canine, and chicken Kaiso (approx.105 kDa).

Immunogen

recombinant purified mouse Kaiso protein (amino acids 1-499).

Application

Anti-Kaiso antibody, Mouse monoclonal may be used in:

• enzyme-linked immunosorbent assay (ELISA)
• immunoblotting
• immunoprecipitation
• immunocytochemistry

Biochem/physiol Actions

Kaiso is a transcriptional repressor that recognizes specifically the DNA sequence TCCTGCNA or two symmetrically methylated CpG dinucleotides. This protein binds to the cell adhesion cofactor p120 ctn which signifies its role in the p120 ctn action and signaling.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.