La disolución salina equilibrada de Hanks se ha utilizado:
en experimentos de inanición para células de mamíferos[1]
para recolectar y mantener piezas cerebrales para el cultivo primario de células gliales[2]
para lavar el tejido femoral y médula ósea (BM) destinados a cultivar células madre mesenquimatosas[3]
Acciones bioquímicas o fisiológicas
La disolución salina equilibrada (BSS) en cultivo celular es multifacética y tiene varias funciones. Actúa como líquido de irrigación, transporte y dilución a la vez que mantiene el equilibrio osmótico intracelular y extracelular. La BSS proporciona a las células agua y ciertos iones inorgánicos a granel esenciales para el metabolismo celular normal. Se combina con carbohidratos como la glucosa para actuar como la principal fuente de energía para el metabolismo celular. La DPBS proporciona también un sistema de amortiguación para mantener el medio dentro del intervalo de pH fisiológico (7,2 -7,6)
Connective tissues (CT) support and connect organs together. Understanding the formation of CT is important, as CT deregulation leads to fibrosis. The identification of CT specific markers has contributed to a better understanding of CT function during development. In developing
Venomous snakes are important subjects of study in evolution, ecology, and biomedicine. Many venomous snakes have alpha-neurotoxins (α-neurotoxins) in their venom. These toxins bind the alpha-1 nicotinic acetylcholine receptor (nAChR) at the neuromuscular junction, causing paralysis and asphyxia. Several venomous
Liver international : official journal of the International Association for the Study of the Liver, 41(1), 206-219 (2020-10-22)
Mature transfer RNAs (tRNA) charged with amino acids decode mRNA to synthesize proteins. Dysregulation of translational machineries has a fundamental impact on cancer biology. This study aims to map the tRNAome landscape in liver cancer patients and to explore potential
Toxicon : official journal of the International Society on Toxinology, 150, 39-49 (2018-05-16)
Currently, biological and organic substances are screened in order to find a new generation of therapeutics active against cancer. Previous research has identified promising candidate peptides in snake venom. In this study, venoms from different snake species (Naja annulifera, Naja
Cancer biology & therapy, 14(2), 135-145 (2012-11-30)
Growing evidence suggests that the cancer stem cell phenotype in melanoma is dynamically regulated. Therefore, effective therapies have to target simultaneously bulk tumor cells and melanoma stem-like cells. The aim of the present study was to investigate the effects of
3D Spheroid BME Cell Invasion Assay utilizes specialized plates and ECM for cell aggregation and spheroid formation in vitro.
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