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H6914

Sigma-Aldrich

Suero humano

(from male AB clotted whole blood), USA origin, sterile-filtered

Sinónimos:

fuera del coágulo, otc, suero humano, sueros humanos

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About This Item

Número MDL:
MFCD00165829
Código UNSPSC:
12352207
NACRES:
NA.71
El producto H6914 no se vende actualmente en su país Póngase en contacto con el Servicio técnico

origen biológico

human

Nivel de calidad

300

esterilidad

sterile-filtered

composición

Hemoglobin, ≤25 mg/dL

origen

USA origin

técnicas

cell culture | mammalian: suitable
cell culture | stem cell: suitable

impurezas

≤10 EU/mL Endotoxin

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

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Aplicación

El suero AB humano se utiliza en aplicaciones de ingeniería genética, trasplantes y terapia celular para la expansión de células madre mesenquimatosas (MSC) del tejido adiposo o las células del estroma mesenquimatoso de la médula ósea humana; para la generación y el trasplante de injertos normalizados de células madre del epitelio del limbo esclero corneal; para la expansión ex vivo de células NK a partir de sangre periférica en medio de expansión de células madre hematopoyéticas; y para actualización de la tecnología de cultivo de islote humano pretrasplante.

Envase

El embalaje puede variar para diferentes lotes debido a la escasez de las botellas utilizadas para embalar este producto. La presencia o falta de envoltura retráctil alrededor de la tapa de la botella no afecta a la integridad/esterilidad del producto.

Advertencia

Se analizó cada donante para la hepatitis B y C y se observó que no eran reactivos , y tampoco lo eran para los anticuerpos del virus de la inmunodeficiencia humana (VIH) mediante ELISA. No obstante, los productos de origen humano deben considerarse potencialmente infecciosos y manejarse en consecuencia.

Nota de análisis

Analizada la presencia de endotoxinas

Otras notas

No es inusual que nuestros productos séricos humanos estén turbios. Este producto procede de la combinación de sueros y la turbidez indica la presencia de lípidos en el suero. No se dispone de productos séricos humanos transparentes, carentes de lípidos.

Cláusula de descargo de responsabilidad

SÓLO PARA INVESTIGACIÓN. Este producto está regulado en Francia cuando está destinado a ser utilizado con fines científicos, como para actividades de importación y exportación (artículo L 1211-1, apartado 2, del Código de Salud Pública). El comprador (es decir, el usuario final) está obligado a obtener una autorización de importación del Ministerio de Investigación de Francia referida al artículo L1245-5-1 II del Código de Salud Pública. Al pedir este producto, usted confirma que ha obtenido la autorización de importación adecuada.

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Referencia del producto
Descripción
Precios

06693

Timestrip Plus™ -20 °C

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number
0000298253
0000298253
0000298252
0000298252
0000343425

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Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

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Serum for Cell and Tissue Culture FAQs

Find answers to frequently asked questions (FAQs) about FBS and other serum products, and links to related products.

Questions

1–9 of 9 Questions  

  1. What is the best way to thaw this product? Once thawed, how long can we use it if kept in at 4 oC?

    1 answer

    1. The recommended storage temperature is -20°C. The following steps confirm the best way to thaw serum:

      1. Remove the serum bottles from the freezer and allow them to acclimate to room temperature for approximately 10 minutes.
      2. Place each container in a 30 to 37 °C water bath or incubator. Excessive temperatures will degrade heat-labile nutrients. If using a
      water bath, prevent the bottle caps from being completely submerged.
      3. Gently swirl or shake the bottles every 10 - 15 minutes until the serum is completely thawed.
      Please note: Multiple thaw/freeze cycles should be avoided as they will hasten serum nutrient degradation and can induce insoluble precipitate formation.

      Helpful?

  2. Is this serum manufactured under cGMP?

    1 answer

    1. This product is not manufactured under cGMP.

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  3. If the serum is being used to complete media for a cell thaw but then the cells are put through a DNA extraction rather than put into culture - would thawing a bottle of serum, aliquoting, and then re-freezing the serum be okay?

    1 answer

    1. Thawed serum should be mixed thoroughly before being added to cell culture medium. Repeated freeze-thaw cycles may significantly impact serum quality. It is recommended to aliquot the thawed serum for single use and store aliquots at -20 °C. If serum is stored between 2-8 °C, it should be used within 2-4 weeks.

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  4. DOES THIS PRODUCT CONTAIN OESTRADIOL

    1 answer

    1. The presence of oestradiol in this product is not determined. To see which compounds are tested for, refer to a sample Certificate of Analysis found at: https://www.sigmaaldrich.com/product/sigma/h6914#product-documentation

      Helpful?

  5. At which biosafety level should Human Serum H6914 be handled?

    1 answer

    1. Blood products should be treated and handled as potentially infectious. It is recommended to use human origin products from normal (non-diseased) donors at Biosafety Level 2 (BSL-2). Although donors may be tested for adventitious infections, as listed on the Certificate of Analysis for the product, no testing can cover all types of infection. Therefore, these products should be treated as potentially infectious and handled accordingly. Laboratory directors and principal investigators are responsible for performing a risk assessment and determining the biosafety level in accordance with applicable national and local regulations.

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  6. Human Serum (Product No. H6914 & Batch No. SLCF0424)의 사용기한은 CoA상에서 2023년 2월 까지인 것으로 확인됩니다. 혹시 해당 batch의 사용기한이 연장될 수도 있을까요?

    1 answer

    1. The shelf life is not extended for those product which are assigned a firm expiration date. No further testing is performed on these lots.

      Helpful?

  7. Are all your human donors consented?

    1 answer

    1. All protocols including donor consent are subject to the strict oversight of an Institutional Review Board (IRB) as governed by US law.

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  8. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  9. What is the difference between human serum from AB plasma and  human serum from AB clotted whole blood?

    1 answer

    1. Product H6914 is prepared from whole blood that is allowed to clot, centrifuged and the serum removed. There is no additional processing required.Product H4522 is prepared from anticoagulated (sodium citrate) whole blood. This is centrifuged to pellet the cells (RBC, WBC, some platelets) and the plasma is removed. This plasma is then treated with calcium to activate the clotting cascade. The preparation is centrifuged again and the supernatant is removed. This product can also be called plasma derived serum.Product H6914 will contain more growth factors since it is allowed to spontaneously clot and there will be the release of growth factors from many of the cells (WBC, platelets) present.  The cells are removed before the plasma is clotted for Product H4522, so this plasma will have fewer growth factors present (more similar to what is circulating). Unless testing for growth factors, most researchers are looking for Product H6914.We offer both products heat inactivated.Product H5667 Human Serum Heat Inactivated, from male AB clotted whole bloodProduct H3667 Human Serum Heat Inactivated, from human male AB plasma

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